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Large Scale Sequence Analysis of Avian Influenza Isolates

Table 3. PDZ binding studies.

Synthetic peptides corresponding to the predominant “avian/equine” PL signature (GSESEV), the predominant “Human” signature, the highly pathogenic (“HPAI”) AIV signature (GSEPEV) and the unique “1918” signature (GSKSEV) were tested for their ability to bind 3 known human PDZ domains by chemical-shift perturbation NMR. The domains represented are the PDZ domain in Disheveled (residues 251-345), the first PDZ in PSD-95 (residues 61-151), and the seventh PDZ in GRIP1 (residues 980-1070). The results are summarized here in terms of relative binding strength. NMR data are shown in Fig. S10.

Dsh ++ ++ ++ -
PSD-95 +++ +\- ++ +\-
GRIP1 - - - -