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Large Scale Sequence Analysis of Avian Influenza Isolates

Figure S11. Testing binding of peptide GSESEV to PDZ domains by NMR.

15N,1H HSQC spectra were collected first with free PDZ domains and additional times after titrating in the peptide. Peaks from spectra with the free PDZ domains are colored blue. Spectra collected after adding increasing concentrations of the peptide are shown sequentially in green, red, and purple peaks. Dosage-dependent chemical shift perturbations among residues within the PDZ domain binding surface indicate binding. Binding to the PDZ domain GRIP1 was negative in all cases and is not shown.

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Figure S11
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