Synthetic peptides corresponding to the predominant “avian/equine” PL signature (GSESEV), the predominant “Human” signature, the highly pathogenic (“HPAI”) AIV signature (GSEPEV) and the unique “1918” signature (GSKSEV) were tested for their ability to bind 3 known human PDZ domains by chemical-shift perturbation NMR. The domains represented are the PDZ domain in Disheveled (residues 251-345), the first PDZ in PSD-95 (residues 61-151), and the seventh PDZ in GRIP1 (residues 980-1070). The results are summarized here in terms of relative binding strength. NMR data are shown in Fig. S10.
| GSESEV (LPAI) |
GSEPEV (HPAI) |
GSKSEV (1918) |
GSRSKV (Human) |
|
|---|---|---|---|---|
| Dsh | ++ | ++ | ++ | - |
| PSD-95 | +++ | +\- | ++ | +\- |
| GRIP1 | - | - | - | - |