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Evans, William, PharmD

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Veerman, Anjo J. P.

St. Jude Research >> AML1 >> SectionII >> Table_s6

Supplementary Information

Gene Expression Profiling of Pediatric Acute Myelogenous Leukemia

Section II: Methods

Table S6. TacMan probes for detection of MLL-PTD

Primer name	Primer direction	Sequence
MLLe	forward	5’-CCTGAATCCAAACAGGCCACCACT-3’
MLL3.1C	reverse	5’-AGGAGAGAGTTTACCTGCTC-3’

Amplification was carried out using cycle parameters of 98^oC for 10 min; 94^oC for 1 min; anneal at 60^oC for 2 min; extend at 72^oC for 2 min for 40 cycles.