Assay Reporter (v1.5) Summary

Project: Anti_Trypanosomal

Protocol: Protocol3C

AssayType: FluorIntensity

Profile Date: 21:7 10.1.2006

Click HERE For Plate List Summary (includes plate stats and heatmaps)
Click HERE For Hit Molecular Profile (Max 300 molecules with Zscore > 1.96)
Click HERE For Hit Activity History (Max 300 molecules with Zscore > 1.96)
Click HERE For Hit Preliminary SAR (Max 300 molecules with Zscore > 1.96)

Please contact Anang Shelat (anang.shelat@stjude.org) if you have problems with this site.

Scatterplot of Activities for all Molecules in Assay

48 Hits out of 2160 (2.2%); Zscore > 1.96 (red line, %Act. > 52.1), Zscore > 2.94 (green line, %Act. > 76.1)

Plates With Poor Z-Prime Values

Signficance Threshold: Z_score > 3.30

refnum ID for group of plates concurrently screened

plateord Order by which the plate was screened

plateid

zfactor A measure of discrimination between positive controls and the compounds in the screen: zfactor < 0.0 indicates a failed assay, 0.0 < zfactor < 0.5 indicates that the assay discriminates between actives and inactives, and 0.5 < zfactor < 1.0 is ideal (see Zhang, J.BioScreening, 1999)

zprime A measure of discrimination between positive and negative controls in the screen: zprime < 0.0 indicates a failed assay, 0.0 < zprime < 0.5 indicates that the assay discriminates between positive and negative controls, and 0.5 < zprime < 1.0 is ideal (see Zhang, J.BioScreening, 1999)

zscore Distance in standard deviations of a sample from the mean; under certain assumptions, a zscore=1.96 corresponds to a p-value of 0.05, and a zscore=3.3 corresponds to a p-value of 0.01

Z-Prime Scatteplot

Z_score > 3.30 (red line), Z_score > 4.95 (green line)

ZPRIMESCATTER

Plates With Poor Z-Factor Values

Signficance Threshold: Z_score > 3.30

refnum ID for group of plates concurrently screened

plateord Order by which the plate was screened

plateid

zfactor A measure of discrimination between positive controls and the compounds in the screen: zfactor < 0.0 indicates a failed assay, 0.0 < zfactor < 0.5 indicates that the assay discriminates between actives and inactives, and 0.5 < zfactor < 1.0 is ideal (see Zhang, J.BioScreening, 1999)

zprime A measure of discrimination between positive and negative controls in the screen: zprime < 0.0 indicates a failed assay, 0.0 < zprime < 0.5 indicates that the assay discriminates between positive and negative controls, and 0.5 < zprime < 1.0 is ideal (see Zhang, J.BioScreening, 1999)

zscore Distance in standard deviations of a sample from the mean; under certain assumptions, a zscore=1.96 corresponds to a p-value of 0.05, and a zscore=3.3 corresponds to a p-value of 0.01

Z-Factor Scatterplot

Z_score > 3.30 (red line), Z_score > 4.95 (green line)

ZFACTORSCATTER

Time Dependent Z-Factors

Average Z-factor over all plates with Confidence Interval

ORDZFACTORPLOT

Well Analysis Across Project

Each well is color coded by its variance Z-score

BLK	-0.35	-0.02	1.29	-1.60	0.33	-1.24	-0.15	0.99	-0.77	-0.88	BLK
BLK	-1.52	-1.31	-1.34	-1.30	-0.10	-1.17	-1.38	0.40	0.18	0.01	BLK
BLK	0.32	0.23	-1.15	0.52	-0.68	-0.62	1.87	1.10	1.04	1.27	BLK
BLK	-1.18	0.96	-0.81	-0.85	-0.47	2.81	-0.61	0.65	0.10	3.14	BLK
BLK	-0.87	-1.01	0.05	-1.11	-0.69	-0.22	1.55	0.54	1.38	-0.37	BLK
BLK	0.01	0.85	-0.51	-0.42	0.36	-0.27	0.01	1.22	0.08	1.27	BLK
BLK	-0.15	1.08	-0.50	0.53	1.32	1.40	0.59	0.67	0.51	0.62	BLK
BLK	-0.80	-0.96	-1.39	0.48	-0.93	-0.92	-1.33	0.50	-0.31	-0.00	BLK

Each well is color coded by its mean Z-score

BLK	1.38	1.21	1.96	0.95	1.15	0.87	0.52	0.58	-0.49	-1.13	BLK
BLK	0.17	0.47	0.58	0.55	0.63	0.64	0.04	0.29	-0.47	-0.07	BLK
BLK	-0.71	-0.86	-0.18	-0.48	-0.53	-1.19	-1.38	-0.99	-1.16	-1.13	BLK
BLK	-0.40	-0.83	-0.52	-1.21	-1.22	-1.04	-1.95	-0.83	-1.49	0.36	BLK
BLK	-0.02	-0.13	-0.71	-1.02	-1.06	-1.55	-1.00	-1.30	-0.65	-1.30	BLK
BLK	0.98	0.40	0.21	-1.07	-0.23	-0.88	-1.39	-0.55	-1.31	-0.62	BLK
BLK	0.94	1.59	1.03	0.76	1.51	0.97	0.71	0.70	0.24	0.02	BLK
BLK	0.69	1.42	1.68	1.96	1.40	1.17	1.19	1.23	1.11	0.80	BLK

Well Analysis By Refnum