St. Jude Research

Assay Reporter (v1.5) Summary

Project: Anti_Malarial2

Protocol: Protocol6B

AssayType: FACS

Profile Date: 0:29 10.2.2006

Click HERE For Plate List Summary (includes plate stats and heatmaps)
Click HERE For Hit Molecular Profile (Max 300 molecules with Zscore > 1.50)
Click HERE For Hit Activity History (Max 300 molecules with Zscore > 1.50)
Click HERE For Hit Preliminary SAR (Max 300 molecules with Zscore > 1.50)

Please contact Anang Shelat (anang.shelat@stjude.org) if you have problems with this site.

Scatterplot of Activities for all Molecules in Assay

228 Hits out of 2160 (10.6%); Zscore > 1.50 (red line, %Act. > 93.7), Zscore > 2.25 (green line, %Act. > 128.9)


ACTSCATTER.png

Plates With Poor Z-Prime Values

Signficance Threshold: Z_score > 3.30


refnum ID for group of plates concurrently screened plateord Order by which the plate was screened plateid zfactor A measure of discrimination between positive controls and the compounds in the screen: zfactor < 0.0 indicates a failed assay, 0.0 < zfactor < 0.5 indicates that the assay discriminates between actives and inactives, and 0.5 < zfactor < 1.0 is ideal (see Zhang, J.BioScreening, 1999) zprime A measure of discrimination between positive and negative controls in the screen: zprime < 0.0 indicates a failed assay, 0.0 < zprime < 0.5 indicates that the assay discriminates between positive and negative controls, and 0.5 < zprime < 1.0 is ideal (see Zhang, J.BioScreening, 1999) zscore Distance in standard deviations of a sample from the mean; under certain assumptions, a zscore=1.96 corresponds to a p-value of 0.05, and a zscore=3.3 corresponds to a p-value of 0.01
20050908124310 1 01833MS -3.33 -2.07 -4.53

Z-Prime Scatteplot

Z_score > 3.30 (red line), Z_score > 4.95 (green line)


ZPRIMESCATTER

Plates With Poor Z-Factor Values

Signficance Threshold: Z_score > 3.30


refnum ID for group of plates concurrently screened plateord Order by which the plate was screened plateid zfactor A measure of discrimination between positive controls and the compounds in the screen: zfactor < 0.0 indicates a failed assay, 0.0 < zfactor < 0.5 indicates that the assay discriminates between actives and inactives, and 0.5 < zfactor < 1.0 is ideal (see Zhang, J.BioScreening, 1999) zprime A measure of discrimination between positive and negative controls in the screen: zprime < 0.0 indicates a failed assay, 0.0 < zprime < 0.5 indicates that the assay discriminates between positive and negative controls, and 0.5 < zprime < 1.0 is ideal (see Zhang, J.BioScreening, 1999) zscore Distance in standard deviations of a sample from the mean; under certain assumptions, a zscore=1.96 corresponds to a p-value of 0.05, and a zscore=3.3 corresponds to a p-value of 0.01
20050908124310 1 01833MS -3.33 -2.07 -3.39

Z-Factor Scatterplot

Z_score > 3.30 (red line), Z_score > 4.95 (green line)


ZFACTORSCATTER

Time Dependent Z-Factors

Average Z-factor over all plates with Confidence Interval


ORDZFACTORPLOT

Well Analysis Across Project

Each well is color coded by its variance Z-score


BLK 0.01 -0.32 0.08 -0.10 2.75 -0.58 0.08 -0.15 -0.37 -0.50 BLK
BLK -0.23 -0.23 -0.25 0.14 -0.12 -0.45 -0.25 -0.18 -0.64 1.44 BLK
BLK -0.04 -0.44 -0.73 6.19 0.26 -0.20 -0.03 -0.20 -0.60 -0.28 BLK
BLK -0.36 3.46 -0.36 -0.84 -0.04 -0.22 -0.61 -0.15 -0.71 -0.10 BLK
BLK -0.65 -0.48 -0.44 -0.30 -0.45 -0.18 0.15 -0.13 -0.17 0.09 BLK
BLK -0.40 0.15 -0.43 -0.27 -0.18 -0.32 -0.39 0.13 -0.27 1.40 BLK
BLK -0.48 -0.33 -0.17 0.12 -0.17 2.31 -0.56 -0.27 -0.14 -0.45 BLK
BLK -0.21 -0.11 0.08 -0.29 -0.51 -0.64 -0.55 -0.11 -0.07 1.56 BLK


Each well is color coded by its mean Z-score


BLK 1.76 0.14 1.06 1.04 -1.74 -0.08 0.79 0.93 0.27 -0.47 BLK
BLK 0.32 -0.32 -0.05 -0.11 0.48 -0.43 0.53 1.44 -0.32 -0.85 BLK
BLK 1.90 0.30 -0.80 -4.48 2.26 0.16 0.52 0.17 -0.50 0.45 BLK
BLK 0.57 -2.37 -0.61 -1.06 0.37 0.49 0.02 0.99 -0.32 0.87 BLK
BLK -0.60 -0.60 -0.51 -0.39 -1.07 -1.21 0.70 1.21 -0.38 2.06 BLK
BLK 0.10 1.06 -0.33 -1.15 -0.95 -0.58 0.08 0.61 0.25 -1.48 BLK
BLK 0.03 0.93 0.99 0.56 -0.56 -0.18 -0.97 1.03 0.72 0.02 BLK
BLK 0.08 0.09 -0.13 -0.28 -0.72 -0.43 -1.09 0.49 0.33 -1.05 BLK


Well Analysis By Refnum

20050908123858 20050908124157 20050908124310 20050922164302