St. Jude Research

Assay Reporter (v1.5) Summary

Project: Anti_Malarial1

Protocol: Protocol6A

AssayType: FACS

Profile Date: 22:15 10.1.2006

Click HERE For Plate List Summary (includes plate stats and heatmaps)
Click HERE For Hit Molecular Profile (Max 300 molecules with Zscore > 1.50)
Click HERE For Hit Activity History (Max 300 molecules with Zscore > 1.50)
Click HERE For Hit Preliminary SAR (Max 300 molecules with Zscore > 1.50)

Please contact Anang Shelat (anang.shelat@stjude.org) if you have problems with this site.

Scatterplot of Activities for all Molecules in Assay

126 Hits out of 2160 (5.8%); Zscore > 1.50 (red line, %Act. > 97.3), Zscore > 2.25 (green line, %Act. > 135.9)


ACTSCATTER.png

Plates With Poor Z-Prime Values

Signficance Threshold: Z_score > 3.30


refnum ID for group of plates concurrently screened plateord Order by which the plate was screened plateid zfactor A measure of discrimination between positive controls and the compounds in the screen: zfactor < 0.0 indicates a failed assay, 0.0 < zfactor < 0.5 indicates that the assay discriminates between actives and inactives, and 0.5 < zfactor < 1.0 is ideal (see Zhang, J.BioScreening, 1999) zprime A measure of discrimination between positive and negative controls in the screen: zprime < 0.0 indicates a failed assay, 0.0 < zprime < 0.5 indicates that the assay discriminates between positive and negative controls, and 0.5 < zprime < 1.0 is ideal (see Zhang, J.BioScreening, 1999) zscore Distance in standard deviations of a sample from the mean; under certain assumptions, a zscore=1.96 corresponds to a p-value of 0.05, and a zscore=3.3 corresponds to a p-value of 0.01
20050908123613 1 01833MS -4.76 -2.28 -4.94

Z-Prime Scatteplot

Z_score > 3.30 (red line), Z_score > 4.95 (green line)


ZPRIMESCATTER

Plates With Poor Z-Factor Values

Signficance Threshold: Z_score > 3.30


refnum ID for group of plates concurrently screened plateord Order by which the plate was screened plateid zfactor A measure of discrimination between positive controls and the compounds in the screen: zfactor < 0.0 indicates a failed assay, 0.0 < zfactor < 0.5 indicates that the assay discriminates between actives and inactives, and 0.5 < zfactor < 1.0 is ideal (see Zhang, J.BioScreening, 1999) zprime A measure of discrimination between positive and negative controls in the screen: zprime < 0.0 indicates a failed assay, 0.0 < zprime < 0.5 indicates that the assay discriminates between positive and negative controls, and 0.5 < zprime < 1.0 is ideal (see Zhang, J.BioScreening, 1999) zscore Distance in standard deviations of a sample from the mean; under certain assumptions, a zscore=1.96 corresponds to a p-value of 0.05, and a zscore=3.3 corresponds to a p-value of 0.01
20050908123613 1 01833MS -4.76 -2.28 -3.77

Z-Factor Scatterplot

Z_score > 3.30 (red line), Z_score > 4.95 (green line)


ZFACTORSCATTER

Time Dependent Z-Factors

Average Z-factor over all plates with Confidence Interval


ORDZFACTORPLOT

Well Analysis Across Project

Each well is color coded by its variance Z-score


BLK 0.16 -0.20 0.12 -0.09 3.72 -0.49 0.17 -0.02 -0.35 -0.58 BLK
BLK -0.26 -0.11 -0.26 -0.01 -0.22 -0.28 -0.23 -0.11 -0.42 1.93 BLK
BLK -0.26 -0.36 -0.31 6.22 -0.01 -0.17 -0.12 -0.24 0.39 -0.16 BLK
BLK -0.17 3.09 -0.41 -1.01 -0.13 -0.35 -0.60 -0.17 -0.46 -0.16 BLK
BLK -0.48 -0.52 -0.51 -0.23 -0.38 -0.26 -0.11 -0.27 -0.21 0.02 BLK
BLK -0.39 -0.11 -0.40 -0.50 -0.23 -0.34 0.92 -0.05 -0.43 1.17 BLK
BLK -0.44 -0.34 -0.20 -0.05 -0.30 1.68 -0.56 -0.37 -0.26 -0.53 BLK
BLK -0.05 -0.28 -0.18 -0.12 -0.32 -0.46 -0.51 0.03 -0.12 -0.40 BLK


Each well is color coded by its mean Z-score


BLK 1.51 0.15 0.71 0.84 -2.58 -0.39 -0.17 0.54 0.26 -0.60 BLK
BLK 0.56 -0.24 0.04 -0.11 0.42 -0.60 0.08 1.05 -0.17 -1.38 BLK
BLK 1.77 0.14 -0.38 -5.25 2.12 0.35 0.50 0.14 -0.63 0.50 BLK
BLK 0.42 -2.43 -0.00 -0.89 0.27 0.83 -0.03 1.28 -0.27 1.57 BLK
BLK 0.11 -0.29 -0.52 0.17 0.05 -0.06 0.67 1.14 -0.12 1.49 BLK
BLK 0.08 1.19 -0.27 -0.68 -0.07 -0.51 -1.03 0.95 0.53 -1.54 BLK
BLK 0.17 0.41 0.47 0.58 0.09 -0.54 -0.51 0.54 0.63 -0.26 BLK
BLK -0.54 0.04 0.25 -0.30 -0.76 -0.63 -0.98 0.29 -0.14 -0.00 BLK


Well Analysis By Refnum

20050908123056 20050908123438 20050908123613 20050922164102