St. Jude Research

RISE (Robust Investigation of Screening Experiments) Home

Protocol Parameters

ScreenRun_ID:MMDMX_02_HEPG2CYTOX_01_DR_01
ScreenRun Comment:1152 HEPG2 Dose-Response Screen
Time Stamp:20090619.024430
Protocol Version:3.01
Data Source:/users/sj_ashelat/Temp/mMDMX_02_HEPG2CYTOX_01_DR/mMDMX_02_HEPG2CYTOX_01_DR_01/Envision_files/777000026506_20080515191331
Cellario Source:/users/sj_ashelat/Temp/mMDMX_02_HEPG2CYTOX_01_DR/mMDMX_02_HEPG2CYTOX_01_DR_01/CELLARIO_SOURCE_TOBOR_465_mMDMX_02_HEPG2CY
Barcode Source:/users/sj_ashelat/Temp/mMDMX_02_HEPG2CYTOX_01_DR/mMDMX_02_HEPG2CYTOX_01_DR_01/mMDMX_02_HEPG2CYTOX_01_DR_01.xls
Assay Readout:ENV_LUMINESCENCE_SP
Activity Measure:POSCTRLs < NEGCTRLs
Endpoint Transform:LOG10
Endpoint Normalization:PRCACT
VARCMPD DR:TRUE
Alternative_Endpoint:NO
Plate Format:384_WELL
Blank Wells:
Reference Wells:A21,B21,C21,D21,E21,F21,G21
Positive Controls:A23,B23,C23,D23,E23,F23,G23,H23,I23,J23,K23,L23,M23,N23,O23,P23
POSCTRL Normalization Wells:A23,B23,C23,D23,E23,F23,G23,H23,I23,J23,K23,L23,M23,N23,O23,P23
Negative Controls:H21,I21,J21,K21,L21,M21,N21,O21,P21,A22,B22,C22,D22,E22,F22,G22,H22,I22,J22,K22,L22,M22,N22,O22,P22,A24,B24,C24,D24,E24,F24,G24,H24,I24,J24,K24,L24,M24,N24,O24,P24
NEGCTRL Normalization Wells:H21,I21,J21,K21,L21,M21,N21,O21,P21,A22,B22,C22,D22,E22,F22,G22,H22,I22,J22,K22,L22,M22,N22,O22,P22,A24,B24,C24,D24,E24,F24,G24,H24,I24,J24,K24,L24,M24,N24,O24,P24
DR Concentration:USE_TEMPLATE
DR Dilution Factor:
DR Sample Layout:USE_REMP
DR Replicates:CORRELATED
DR RobustFit:FALSE
MinMinMax Range:25,50,150
MinMax HillSlope:0.50,25.0
MinMax EndSlope:0.20,0.75
MinMax Fold EC50 CI:5,10
Retrieve Structure:YES
Generate Platemaps:NO
Excluded Plates: 777000049871 777000049888 777000049550

RISE Warnings

NONE

Quality Control Assessment

Assay - Compound Plate Reconciliation

Cellario Time Analysis

Preprocessing Analysis

Endpoint Analysis

Assay Activity Summary

# Processed Plates# Processed Data Points# Compounds Screened# HitsHit Rate
108414721152140 0.12153

Compound TypeConsensus Curve Class# Unique CompoundsModel SummaryDetailed Text
References and ControlsMixed Classes108 Link Link
Variable CompoundsA1109 Link Link
Variable CompoundsB131 Link Link
Variable CompoundsC1279 Link Link
Variable CompoundsD1732 Link Link
Variable CompoundsNA10 NONENONE
Variable CompoundsNB10 NONENONE
Variable CompoundsNC11 Link Link

Potency Group# Unique CompoundsSummary Table
HIGH46 Link
MED46 Link
LOW48 Link
UND280 Link
INACTIVE732 Link

Download all Compound Consensus Data

Archive Files

CHEMBIO Archive File: RISE_20090619024430_MMDMX_02_HEPG2CYTOX_01_DR_01.tar.gz
WRAP Input File: WRAP_20090619024430_MMDMX_02_HEPG2CYTOX_01_DR_01.tar.gz
Archival Instructions: Instructions for extracting the CHEMBIO archive file are HERE. Please download and extract the CHEMBIO archive file, then copy the extract folder to the appropriate Target folder on \\linux-file1\chembio. The WRAP input file should be loaded into the WRAP protocol without modification.

Cheminformatics Datafiles

Processed Cellario Data file

ScreenRun Information file

Processed Assay Data file

Processed Plate Data file

All Dose Response Model Data file

Consensus Dose Response Model Data file