St. Jude Research

RISE (Robust Investigation of Screening Experiments) Home

Protocol Parameters

ScreenRun_ID:MMDMX_02_BJCYTOX_01_DR_01
ScreenRun Comment:1152 BJ Dose-Response Screen
Time Stamp:20090619.012756
Protocol Version:3.01
Data Source:/users/sj_ashelat/Temp/mMDMX_02_BJCYTOX_01_DR/mMDMX_02_BJCYTOX_01_DR_01/Envision_files/777000044005_20080519103609.txt,/
Cellario Source:/users/sj_ashelat/Temp/mMDMX_02_BJCYTOX_01_DR/mMDMX_02_BJCYTOX_01_DR_01/CELLARIO_SOURCE_TOBOR_471_mMDMX_02_BJCYTO_01_DR.
Barcode Source:/users/sj_ashelat/Temp/mMDMX_02_BJCYTOX_01_DR/mMDMX_02_BJCYTOX_01_DR_01/mMDMX_02_BJCYTOX_01_DR_01.xls
Assay Readout:ENV_LUMINESCENCE_SP
Activity Measure:POSCTRLs < NEGCTRLs
Endpoint Transform:LOG10
Endpoint Normalization:PRCACT
VARCMPD DR:TRUE
Alternative_Endpoint:NO
Plate Format:384_WELL
Blank Wells:
Reference Wells:A21,B21,C21,D21,E21,F21,G21
Positive Controls:A23,B23,C23,D23,E23,F23,G23,H23,I23,J23,K23,L23,M23,N23,O23,P23
POSCTRL Normalization Wells:A23,B23,C23,D23,E23,F23,G23,H23,I23,J23,K23,L23,M23,N23,O23,P23
Negative Controls:H21,I21,J21,K21,L21,M21,N21,O21,P21,A22,B22,C22,D22,E22,F22,G22,H22,I22,J22,K22,L22,M22,N22,O22,P22,A24,B24,C24,D24,E24,F24,G24,H24,I24,J24,K24,L24,M24,N24,O24,P24
NEGCTRL Normalization Wells:H21,I21,J21,K21,L21,M21,N21,O21,P21,A22,B22,C22,D22,E22,F22,G22,H22,I22,J22,K22,L22,M22,N22,O22,P22,A24,B24,C24,D24,E24,F24,G24,H24,I24,J24,K24,L24,M24,N24,O24,P24
DR Concentration:USE_TEMPLATE
DR Dilution Factor:
DR Sample Layout:USE_REMP
DR Replicates:CORRELATED
DR RobustFit:FALSE
MinMinMax Range:25,50,150
MinMax HillSlope:0.50,25.0
MinMax EndSlope:0.20,0.75
MinMax Fold EC50 CI:5,10
Retrieve Structure:YES
Generate Platemaps:NO
Excluded Plates: 777000046863 777000044395 777000044036

RISE Warnings

NONE

Quality Control Assessment

Assay - Compound Plate Reconciliation

Cellario Time Analysis

Preprocessing Analysis

Endpoint Analysis

Assay Activity Summary

# Processed Plates# Processed Data Points# Compounds Screened# HitsHit Rate
108414721152100 0.08681

Compound TypeConsensus Curve Class# Unique CompoundsModel SummaryDetailed Text
References and ControlsMixed Classes108 Link Link
Variable CompoundsA173 Link Link
Variable CompoundsB127 Link Link
Variable CompoundsC1121 Link Link
Variable CompoundsD1872 Link Link
Variable CompoundsNA11 Link Link
Variable CompoundsNB10 NONENONE
Variable CompoundsNC158 Link Link

Potency Group# Unique CompoundsSummary Table
HIGH15 Link
MED52 Link
LOW34 Link
UND179 Link
INACTIVE872 Link

Download all Compound Consensus Data

Archive Files

CHEMBIO Archive File: RISE_20090619012756_MMDMX_02_BJCYTOX_01_DR_01.tar.gz
WRAP Input File: WRAP_20090619012756_MMDMX_02_BJCYTOX_01_DR_01.tar.gz
Archival Instructions: Instructions for extracting the CHEMBIO archive file are HERE. Please download and extract the CHEMBIO archive file, then copy the extract folder to the appropriate Target folder on \\linux-file1\chembio. The WRAP input file should be loaded into the WRAP protocol without modification.

Cheminformatics Datafiles

Processed Cellario Data file

ScreenRun Information file

Processed Assay Data file

Processed Plate Data file

All Dose Response Model Data file

Consensus Dose Response Model Data file