St. Jude Research

RISE (Robust Investigation of Screening Experiments) Home

Protocol Parameters

ScreenRun_ID:MMDMX_02_HMDMX_01_DR_01
ScreenRun Comment:1152 HMDMX Dose-Response Screen
Time Stamp:20090618.221640
Protocol Version:3.01
Data Source:/users/sj_ashelat/Temp/mMDMX_02_hMDMX_01_DR/mMDMX_02_hMDMX_01_DR_01/Envision_files/200000031398_20080319194211.txt,/user
Cellario Source:/users/sj_ashelat/Temp/mMDMX_02_hMDMX_01_DR/mMDMX_02_hMDMX_01_DR_01/CELLARIO_SOURCE_TOBOR_413_mMDMX_02_hMDMX_01_DR.txt
Barcode Source:/users/sj_ashelat/Temp/mMDMX_02_hMDMX_01_DR/mMDMX_02_hMDMX_01_DR_01/mMDMX_02_hMDMX_01_DR_01.xls
Assay Readout:ENV_FPOLAR_SP
Activity Measure:POSCTRLs < NEGCTRLs
Endpoint Transform:NONE
Endpoint Normalization:PRCACT
VARCMPD DR:TRUE
Alternative_Endpoint:NO
Plate Format:384_WELL
Blank Wells:
Reference Wells:A21,B21,C21,D21,E21,F21,G21,H21,A22,B22,C22,D22,E22,F22,G22,H22
Positive Controls:A24,B24,C24,D24,E24,F24,G24,H24,I24,J24,K24,L24,M24,N24,O24,P24
POSCTRL Normalization Wells:A24,B24,C24,D24,E24,F24,G24,H24,I24,J24,K24,L24,M24,N24,O24,P24
Negative Controls:I21,J21,K21,L21,M21,N21,O21,P21,I22,J22,K22,L22,M22,N22,O22,P22,A23,B23,C23,D23,E23,F23,G23,H23,I23,J23,K23,L23,M23,N23,O23,P23
NEGCTRL Normalization Wells:I21,J21,K21,L21,M21,N21,O21,P21,I22,J22,K22,L22,M22,N22,O22,P22,A23,B23,C23,D23,E23,F23,G23,H23,I23,J23,K23,L23,M23,N23,O23,P23
DR Concentration:USE_TEMPLATE
DR Dilution Factor:
DR Sample Layout:USE_REMP
DR Replicates:CORRELATED
DR RobustFit:FALSE
MinMinMax Range:25,50,150
MinMax HillSlope:0.50,25.0
MinMax EndSlope:0.20,0.75
MinMax Fold EC50 CI:5,10
Retrieve Structure:YES
Generate Platemaps:NO
Excluded Plates: 200000074395 200000042059 200000042066

RISE Warnings

NONE

Quality Control Assessment

Assay - Compound Plate Reconciliation

Cellario Time Analysis

Preprocessing Analysis

Endpoint Analysis

Assay Activity Summary

# Processed Plates# Processed Data Points# Compounds Screened# HitsHit Rate
108414721152702 0.60938

Compound TypeConsensus Curve Class# Unique CompoundsModel SummaryDetailed Text
References and ControlsMixed Classes108 Link Link
Variable CompoundsA1346 Link Link
Variable CompoundsB1356 Link Link
Variable CompoundsC1192 Link Link
Variable CompoundsD1253 Link Link
Variable CompoundsNA12 Link Link
Variable CompoundsNB10 NONENONE
Variable CompoundsNC13 Link Link

Potency Group# Unique CompoundsSummary Table
HIGH230 Link
MED233 Link
LOW241 Link
UND195 Link
INACTIVE253 Link

Download all Compound Consensus Data

Archive Files

CHEMBIO Archive File: RISE_20090618221640_MMDMX_02_HMDMX_01_DR_01.tar.gz
WRAP Input File: WRAP_20090618221640_MMDMX_02_HMDMX_01_DR_01.tar.gz
Archival Instructions: Instructions for extracting the CHEMBIO archive file are HERE. Please download and extract the CHEMBIO archive file, then copy the extract folder to the appropriate Target folder on \\linux-file1\chembio. The WRAP input file should be loaded into the WRAP protocol without modification.

Cheminformatics Datafiles

Processed Cellario Data file

ScreenRun Information file

Processed Assay Data file

Processed Plate Data file

All Dose Response Model Data file

Consensus Dose Response Model Data file