St. Jude Research

RISE (Robust Investigation of Screening Experiments) Home

Protocol Parameters

ScreenRun_ID:MMDMX_02_DR_01
ScreenRun Comment:1152 MMMDX Dose-Response Screen
Time Stamp:20090618.150304
Protocol Version:3.01
Data Source:/users/sj_ashelat/Temp/mMDMX_02_DR/mMDMX_02_DR_01/Envision_Files/200000027612_20080324154137.txt,/users/sj_ashelat/Temp/
Cellario Source:/users/sj_ashelat/Temp/mMDMX_02_DR/mMDMX_02_DR_01/CELLARIO_TOBOR_415_mMDMX_01_DR.txt
Barcode Source:/users/sj_ashelat/Temp/mMDMX_02_DR/mMDMX_02_DR_01/mMDMX_02_DR_01.xls
Assay Readout:ENV_FPOLAR_SP
Activity Measure:POSCTRLs < NEGCTRLs
Endpoint Transform:NONE
Endpoint Normalization:PRCACT
VARCMPD DR:TRUE
Alternative_Endpoint:NO
Plate Format:384_WELL
Blank Wells:
Reference Wells:A21,B21,C21,D21,E21,F21,G21,H21,A22,B22,C22,D22,E22,F22,G22,H22
Positive Controls:A24,B24,C24,D24,E24,F24,G24,H24,I24,J24,K24,L24,M24,N24,O24,P24
POSCTRL Normalization Wells:A24,B24,C24,D24,E24,F24,G24,H24,I24,J24,K24,L24,M24,N24,O24,P24
Negative Controls:I21,J21,K21,L21,M21,N21,O21,P21,I22,J22,K22,L22,M22,N22,O22,P22,A23,B23,C23,D23,E23,F23,G23,H23,I23,J23,K23,L23,M23,N23,O23,P23
NEGCTRL Normalization Wells:I21,J21,K21,L21,M21,N21,O21,P21,I22,J22,K22,L22,M22,N22,O22,P22,A23,B23,C23,D23,E23,F23,G23,H23,I23,J23,K23,L23,M23,N23,O23,P23
DR Concentration:USE_TEMPLATE
DR Dilution Factor:
DR Sample Layout:USE_REMP
DR Replicates:CORRELATED
DR RobustFit:FALSE
MinMinMax Range:25,50,150
MinMax HillSlope:0.50,25.0
MinMax EndSlope:0.20,0.75
MinMax Fold EC50 CI:5,10
Retrieve Structure:YES
Generate Platemaps:NO
Excluded Plates: 200000073701 200000027940 200000042707

RISE Warnings

NONE

Quality Control Assessment

Assay - Compound Plate Reconciliation

Cellario Time Analysis

Preprocessing Analysis

Endpoint Analysis

Assay Activity Summary

# Processed Plates# Processed Data Points# Compounds Screened# HitsHit Rate
108414721152334 0.28993

Compound TypeConsensus Curve Class# Unique CompoundsModel SummaryDetailed Text
References and ControlsMixed Classes108 Link Link
Variable CompoundsA1124 Link Link
Variable CompoundsB1210 Link Link
Variable CompoundsC1433 Link Link
Variable CompoundsD1356 Link Link
Variable CompoundsNA113 Link Link
Variable CompoundsNB11 Link Link
Variable CompoundsNC115 Link Link

Potency Group# Unique CompoundsSummary Table
HIGH107 Link
MED118 Link
LOW123 Link
UND448 Link
INACTIVE356 Link

Download all Compound Consensus Data

Archive Files

CHEMBIO Archive File: RISE_20090618150304_MMDMX_02_DR_01.tar.gz
WRAP Input File: WRAP_20090618150304_MMDMX_02_DR_01.tar.gz
Archival Instructions: Instructions for extracting the CHEMBIO archive file are HERE. Please download and extract the CHEMBIO archive file, then copy the extract folder to the appropriate Target folder on \\linux-file1\chembio. The WRAP input file should be loaded into the WRAP protocol without modification.

Cheminformatics Datafiles

Processed Cellario Data file

ScreenRun Information file

Processed Assay Data file

Processed Plate Data file

All Dose Response Model Data file

Consensus Dose Response Model Data file