St. Jude Research/Supplemental Data/vex-vncRS

Supplementary Information for:
Revising the Role of the Pneumococcal vex-vncRS Locus and vancomycin resistance.
Wolfgang Haas¹, Jack E. Sublett¹, Deepak Kaushal² and Elaine I. Tuomanen^1#. J. Bacteriol. Vol. 186. 2004.

¹ Department of Infectious Diseases, St Jude Children's Research Hospital
² Hartwell Center for Bioinformatics and Biotechnology, St Jude Children's Research Hospital
^# Corresponding author

Supplementary Table – Primers used for cloning and PCR-ligation mutagenesis

Amplicon	PCR primers
ermB	5’-AGTCCCGGGAATAAGACTTAGAAGCAAACTTAAGAGTGT 5’-CCTGCAGGGCCCGACATTTATTTGTATAGTTCATCCA 5’-TTCCAAATTTACAAAAGCGACTCA 5’-AATAAGACTTAGAAGAAACTTAAGAGTGT
5’ of vex3	5’-AGGCAAGAGCTCGATCTTCCTA 5’-AACGCCGGCTCCCTTTCTAGATTTTGTTTTAATCA 5’-GAGTCGCTTTTGTAAATTTGGAATCCCTTTCTAGATTTTGT TTTAATCA
3’ of vex3	5’-AAGCGGCCGTGCTGGATGGTGAATAAATTTGAA 5’-CATTGCGCACAGTGAGGATACA 5’-ACACTCTTAAGTTTCTTCTAAGTCTTATTTGCTGGA TGGTGAATAAATTTGAA
5’ of pep₂₇	5’-CTAGTCGAAGGAGAGCACTTAACC 5’-TATGCCGGCGCACTAAAGTACAAAGAAAGCAACTG
3’ of pep₂₇	5’-AAACGGCCGGCAAGAGACTATAATAGAAAATAGGG 5’-AACTTGTCCTCGGTCATCTCAC
5’ of vncR	5’-CACTCGTCAAGAGCTCCTCTAAC 5’-TTTGCCGGCTTTAACTGCTCTCTATTTTTTATTTTTC
3’ of vncR	5’-TCACGGCCGTGCGGAAAAAGCTAGACTTGGA 5’-ACTAAGCCACCTGGAACAGAGT
5’ of vncS	5’-TTGGTGGTGGTGCAGAAGTAGA 5’-GTTGCCGGCTCGCTCCAATTTATAACCAACA 5’-TGAGTCGCTTTTGTAAATTTGGAAGAAGGTATAGAT AAATATCTTTGCAA
3’ of vncS	5’-CTTCGGCCGAGATTTTCCAAAAGTCGTCCAAGA 5’-TACGAGTTGCGTTAGCGAATGC 5’-ACACTCTTAAGTTTCTTCTAAGTCTTATTAGATTTT CCAAAAGTCGTCCAAGA
5’ of lytA	5’-AGCTTGTACGCGGATCTCTTGG 5’-AATTGCCGGCTTCTACTCCTTATCAATTAAAACAACTCA 5’-TGAGTCGCTTTTGTAAATTTGGAATTCTACTCCTTATCAAT TAAAACAACTCA
3’ of lytA	5’-AGACGGCCGGATTACAGTAAAATAATAATGGAATGTC 5’-CTATCGGCGATTCACCATCAGC 5’-ACACTCTTAAGTTTCTTCTAAGTCTTATTGATTACAGTA AAATAATAATGGAATGTC
5’ of SP2155	5’-ATGCTCTTCGCTCTTCTGTTCC 5’-TCAGCCGGCCTCCCATCATTCATTATTACTGTATATTT
3’ of SP2155	5’-AACCGGCCGAATCGATCAGGACAGTCAAATC 5’-GTCAAGACGGCACAGGAAGAC
fcsK promoter	5’-AAGCGTCGACATATTGAAAAAATAAAACGTGG 5’-CGCTTCCATATGTCTCCTCCCTTCGTCCTTG
vncR gene	5’-CAAGGACGAAGGGAGGAGACATATGAAAATTTTAAT TGTAGAAGATGAAGAGATGATC 5’-GCAGGGATCCAGAACACCATATGTCGAGAAGGTATAGAT