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Supplementary Information for:
Vancomycin stress response in a sensitive and a tolerant strain of Streptococcus pneumoniae
Wolfgang Haas1, Deepak Kaushal2, Jack Sublett1, Caroline Obert1 and Elaine I. Tuomanen1
Journal of Bacteriology, In Press
1 Infectious Diseases and 2 Hartwell Center for Bioinformatics and Biotechnology, St Jude Children's Research Hospital. |
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Three replicate experiments were performed for each time point. Two replicate experiments were performed with RNA from untreated cells labeled with Cy3 and RNA from Vancomycin-treated cells labeled with Cy5 dye. The third replicate experiment was a dye-flip (untreated RNA labeled with Cy5 and Vancomycin-treated RNA labeled with Cy3).
T4 (Sensitive strain) + Vancomycin stress for 10 min: |
| 12462818v41.gpr (replicate 1) |
3.5MB |
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| 12469481v41.gpr (replicate 2) |
3.4MB |
FTP |
HTTP |
| 12469479v41.gpr (replicate 3 with dye-flip) |
3.4MB |
FTP |
HTTP |
| T4 (Sensitive strain) + Vancomycin stress for 20 min: |
| 12611895v41.gpr (replicate 1) |
3.4MB |
FTP |
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| 12611896v41.gpr (replicate 2) |
3.4MB |
FTP |
HTTP |
| 12613344v41.gpr (replicate 3 with dye-flip) |
3.5MB |
FTP |
HTTP |
| Tupelo (tolerant strain) + Vancomycin stress for 10 min: |
| 12613410v41.gpr (replicate 1) |
3.4MB |
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| 12611531v41.gpr (replicate 2) |
3.2MB |
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| 12611530v41.gpr (replicate 3 with dye-flip) |
3.2MB |
FTP |
HTTP |
| Tupelo (tolerant strain) + Vancomycin stress for 20 min: |
| 12611535v41.gpr (replicate 1) |
3.3MB |
FTP |
HTTP |
| 12613409v41.gpr (replicate 2) |
3.3MB |
FTP |
HTTP |
| 12613411v41.gpr (replicate 3 with dye-flip) |
3.3MB |
FTP |
HTTP |
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