T-statistics
T-statistics is a classical feature selection approach. The t-statistics of a gene is defined as T = |μ₁ - μ₂|/sqrt(σ₁²/n₁ + σ₂²/n₂), where μ_i is the mean expression of that gene in the i^th class, σ_i² is the variance of that gene in the i^th class and n_i is the size of the i^th class. This formula assigns higher value to a gene that has larger mean difference between two classes and has smaller variance within both classes. Data was log transformed prior to the application of the metric. The 40 top ranked genes for each diagnostic group are listed in Table 13. Generally, using the top 20-40 genes did not result in significant changes to subtype prediction accuracy. So we used only the top 20 genes for subtype prediction, unless noted otherwise.

Table 13. Genes Selected by T statistics


	Affymetrix number	Gene Name	Gene Symbol	Reference number	T-stat value	Above/ Below Mean
*BCR-ABL*
1	32319_at	tumor necrosis factor ligand superfamily member 4 tax-transcriptionally activated glycoprotein 1 34kD	TNFSF4	AL022310	12.0346	Above
2	36194_at	low density lipoprotein-related protein-associated protein 1 alpha-2-macroglobulin receptor-associated protein 1	LRPAP1	M63959	-11.3077	Below
3	1211_s_at	CASP2 and RIPK1 domain containing adaptor with death domain	CRADD	U84388	10.6627	Above
4	37397_at	Homo sapiens platelet/endothelial cell adhesion molecule-1 (PECAM-1) gene, exon 16 and complete cds.	PECAM	L34657	10.2460	Above
5	330_s_at	tubulin, alpha 1, isoform 44	TUBA1	HG2259-HT2348	10.0540	Above
6	33774_at	caspase 8 apoptosis-related cysteine protease	CASP8	X98172	9.9147	Above
7	202_at	heat shock transcription factor 2	HSF2	M65217	-9.7639	Below
8	1558_g_at	p21/Cdc42/Rac1-activated kinase 1 yeast Ste20-related	PAK1	U24152	9.6562	Above
9	39691_at	SH3-containing protein SH3GLB1	SH3GLB1	AB007960	9.5307	Above
10	2045_s_at	hemopoietic cell kinase	HCK	M16592	-9.3898	Below
11	36591_at	tubulin alpha 1 testis specific	TUBA1	X06956	9.3382	Above
12	1386_at	protein tyrosine phosphatase non-receptor type 9	PTPN9	M83738	-9.2414	Below
13	35991_at	Sm protein F	LSM6	AA917945	9.0298	Above
14	41273_at	FK506 binding protein 12-rapamycin associated protein 1	FRAP1	AL046940	8.9732	Above
15	35970_g_at	M-phase phosphoprotein 9	MPHOSPH9	N23137	8.6474	Above
16	38636_at	immunoglobulin superfamily containing leucine-rich repeat	ISLR	AB003184	8.4291	Above
17	36683_at	matrix Gla protein	MGP	AI953789	-8.3872	Below
18	39070_at	singed Drosophila like sea urchin fascin homolog like	SNL	U03057	8.2583	Above
19	40798_s_at	a disintegrin and metalloproteinase domain 10	ADAM10	Z48579	8.2283	Above
20	41649_at	FOXJ2 forkhead factor	LOC55810	AF038177	8.2275	Above
21	38966_at	glycoprotein synaptic 2	GPSN2	AF038958	8.2080	Above
22	34759_at	Human hbc647 mRNA sequence	�	U68494	8.1863	Above
23	1434_at	phosphatase and tensin homolog mutated in multiple advanced cancers 1	PTEN	U92436	8.1671	Above
24	40167_s_at	CS box-containing WD protein	LOC55884	AF038187	8.1655	Above
25	40264_g_at	zinc finger protein-like 1	ZFPL1	AF001891	8.1384	Above
26	36129_at	KIAA0397 gene product	KIAA0397	AB007857	8.0041	Above
27	551_at	E1A binding protein p300	EP300	U01877	-7.7578	Below
28	38345_at	centrosomal protein 1	CEP1	AF083322	-7.7431	Below
29	41137_at	myosin phosphatase target subunit 2	MYPT2	AB007972	-7.7301	Below
30	39068_at	protein phosphatase 2 regulatory subunit B B56 delta isoform	PPP2R5D	L76702	-7.6161	Below
31	38160_at	lymphocyte antigen 75	LY75	AF011333	7.5830	Above
32	34314_at	ribonucleotide reductase M1 polypeptide	RRM1	X59543	7.5778	Above
33	39519_at	KIAA0692 protein	KIAA0692	AB014592	7.4662	Above
34	32788_at	RAN binding protein 2	RANBP2	D42063	7.4114	Above
35	34882_at	nucleolar protein KKE/D repeat	NOP56	Y12065	7.3622	Above
36	2064_g_at	excision repair cross-complementing rodent repair deficiency complementation group 5	ERCC5	L20046	7.3597	Above
37	41836_at	protein with polyglutamine repeat calcium ca2 homeostasis endoplasmic reticulum protein	ERPROT213-21	U94836	7.3350	Above
38	1563_s_at	tumor necrosis factor receptor superfamily member 1A	TNFRSF1A	M58286	7.3039	Above
39	37047_at	Niemann-Pick disease type C1	NPC1	AF002020	7.2357	Above
40	32724_at	phytanoyl-CoA hydroxylase Refsum disease	PHYH	AF023462	-7.2252	Below

*E2A-PBX1*
1	32063_at	pre-B-cell leukemia transcription factor 1	PBX1	M86546	126.7442	Above
2	33355_at	Homo sapiens cDNA FLJ12900 fis clone NT2RP2004321 (by CELERA search of target sequence = PBX1)	PBX1	AL049381	36.6116	Above
3	40454_at	FAT tumor suppressor Drosophila homolog	FAT	X87241	30.7577	Above
4	717_at	GS3955 protein	GS3955	D87119	23.7813	Above
5	39070_at	singed Drosophila like sea urchin fascin homolog like	SNL	U03057	-22.8956	Below
6	33641_g_at	nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1	NFKBIL1	Y14768	-20.4637	Below
7	36536_at	schwannomin interacting protein 1	SCHIP-1	AF070614	-20.1554	Below
8	854_at	B lymphoid tyrosine kinase	BLK	S76617	19.6467	Above
9	37625_at	interferon regulatory factor 4	IRF4	U52682	18.8419	Above
10	39614_at	KIAA0802 protein	KIAA0802	AB018345	17.8214	Above
11	37099_at	arachidonate 5-lipoxygenase-activating protein	ALOX5AP	AI806222	-17.7944	Below
12	38994_at	STAT induced STAT inhibitor-2	STATI2	AF037989	-17.6553	Below
13	37641_at	Human gene for hepatitis C-associated microtubular aggregate protein p44, exon 9 and complete cds.	�	D28915	-17.3074	Below
14	40113_at	GS3955 protein	GS3955	D87119	16.7288	Above
15	2031_s_at	cyclin-dependent kinase inhibitor 1A p21 Cip1	CDKN1A	U03106	-14.9826	Below
16	330_s_at	tubulin, alpha 1, isoform 44	TUBA1	HG2259-HT2348	-14.8016	Below
17	38340_at	huntingtin interacting protein-1-related	KIAA0655	AB014555	14.7180	Above
18	38510_at	Homo sapiens mRNA cDNA DKFZp586B0220	�	AL049435	-14.4522	Below
19	268_at	Homo sapiens platelet/endothelial cell adhesion molecule-1 (PECAM-1) gene, exon 16 and complete cds.	PECAM	L34657	-13.7540	Below
20	2062_at	insulin-like growth factor binding protein 7	IGFBP7	L19182	13.6403	Above
21	37893_at	protein tyrosine phosphatase non-receptor type 2	PTPN2	AI828880	13.5099	Above
22	38580_at	guanine nucleotide binding protein G protein q polypeptide	GNAQ	U43083	-12.8525	Below
23	40049_at	death-associated protein kinase 1	DAPK1	X76104	-12.3837	Below
24	38393_at	KIAA0247 gene product	KIAA0247	D87434	12.3436	Above
25	39379_at	Homo sapiens mRNA cDNA DKFZp586C1019	�	AL049397	12.2102	Above
26	430_at	nucleoside phosphorylase	NP	X00737	12.1307	Above
27	37975_at	cytochrome b-245 beta polypeptide chronic granulomatous disease	CYBB	X04011	-12.0743	Below
28	34862_at	CGI-49 protein	LOC51097	AA005018	12.0264	Above
29	39756_g_at	X-box binding protein 1	XBP1	Z93930	-11.9796	Below
30	307_at	arachidonate 5-lipoxygenase	ALOX5	J03600	-11.9492	Below
31	37304_at	chromobox homolog 1 Drosophila HP1 beta	CBX1	U35451	11.9422	Above
32	1287_at	ADP-ribosyltransferase NAD poly ADP-ribose polymerase	ADPRT	J03473	11.9051	Above
33	1520_s_at	interleukin 1 beta	IL1B	X04500	11.7327	Above
34	596_s_at	colony stimulating factor 3 receptor granulocyte	CSF3R	M59820	-11.6814	Below
35	37493_at	colony stimulating factor 2 receptor beta low-affinity granulocyte-macrophage	CSF2RB	H04668	11.6620	Above
36	36452_at	synaptopodin	KIAA1029	AB028952	11.4021	Above
37	1081_at	ornithine decarboxylase 1	ODC1	M33764	11.2865	Above
38	1563_s_at	tumor necrosis factor receptor superfamily member 1A	TNFRSF1A	M58286	-11.1361	Below
39	39069_at	AE-binding protein 1	AEBP1	AF053944	11.0984	Above
40	36203_at	ornithine decarboxylase 1	ODC1	X16277	10.9475	Above

*Hyperdiploid >50*
1	36620_at	superoxide dismutase 1 soluble amyotrophic lateral sclerosis 1 adult	SOD1	X02317	9.1574	Above
2	39878_at	protocadherin 9	PCDH9	AI524125	-6.9008	Below
3	37543_at	Rac/Cdc42 guanine exchange factor GEF 6	ARHGEF6	D25304	6.8366	Above
4	41470_at	prominin mouse like 1	PROML1	AF027208	6.7290	Above
5	31492_at	muscle specific gene	M9	AB019392	-6.6885	Below
6	38968_at	SH3-domain binding protein 5 BTK-associated	SH3BP5	AB005047	6.4051	Above
7	1915_s_at	v-fos FBJ murine osteosarcoma viral oncogene homolog	FOS	V01512	6.4008	Above
8	37677_at	phosphoglycerate kinase 1	PGK1	V00572	6.2865	Above
9	39867_at	Tu translation elongation factor mitochondrial	TUFM	S75463	-6.2299	Below
10	36795_at	prosaposin variant Gaucher disease and variant metachromatic leukodystrophy	PSAP	J03077	6.1812	Above
11	40875_s_at	small nuclear ribonucleoprotein 70kD polypeptide RNP antigen	SNRP70	X06815	-6.0877	Below
12	306_s_at	high-mobility group nonhistone chromosomal protein 14	HMG14	J02621	6.0804	Above
13	41724_at	accessory proteins BAP31/BAP29	DXS1357E	X81109	6.0244	Above
14	39168_at	Ac-like transposable element	ALTE	AB018328	5.9336	Above
15	955_at	calmodulin type I	CALM1	HG1862-HT1897	5.8650	Above
16	38604_at	neuropeptide Y	NPY	AI198311	5.8313	Above
17	39147_g_at	alpha thalassemia/mental retardation syndrome X-linked RAD54 S. cerevisiae homolog	ATRX	U72936	5.8181	Above
18	39069_at	AE-binding protein 1	AEBP1	AF053944	-5.6901	Below
19	37014_at	myxovirus influenza resistance 1 homolog of murine interferon-inducible protein p78	MX1	M33882	5.6688	Above
20	1520_s_at	interleukin 1 beta	IL1B	X04500	5.6605	Above
21	1488_at	protein tyrosine phosphatase receptor type K	PTPRK	L77886	-5.5877	Below
22	32553_at	MYC-associated zinc finger protein purine-binding transcription factor	MAZ	M94046	-5.5000	Below
23	36169_at	NADH dehydrogenase ubiquinone 1 alpha subcomplex 1 7.5kD MWFE	NDUFA1	N47307	5.4376	Above
24	1817_at	prefoldin 5	PFDN5	D89667	-5.4110	Below
25	578_at	Human recombination acitivating protein (RAG2) gene, last exon	RAG2	M94633	-5.4026	Below
26	1556_at	RNA binding motif protein 5	RBM5	U23946	-5.3032	Below
27	40998_at	trinucleotide repeat containing 11 THR-associated protein 230 kDa subunit	TNRC11	AF071309	5.2349	Above
28	37294_at	B-cell translocation gene 1 anti-proliferative	BTG1	X61123	-5.1877	Below
29	1447_at	proteasome prosome macropain subunit beta type 1	PSMB1	D00761	5.1699	Above
30	35940_at	POU domain class 4 transcription factor 1	POU4F1	X64624	5.1200	Above
31	33307_at	kraken-like	BK126B4.1	AL022316	-5.0984	Below
32	1081_at	ornithine decarboxylase 1	ODC1	M33764	-5.0822	Below
33	34336_at	lysyl-tRNA synthetase	KARS	D32053	-5.0692	Below
34	41143_at	Human calmodulin (CALM1) gene, exons 2,3,4,5 and 6, and complete cds	CALM1	U12022	5.0543	Above
35	32251_at	hypothetical protein FLJ21174	FLJ21174	AA149307	5.0373	Above
36	35298_at	eukaryotic translation initiation factor 3 subunit 7 zeta 66/67kD	EIF3S7	U54558	-4.9499	Below
37	38649_at	KIAA0970 protein	KIAA0970	AB023187	-4.9228	Below
38	36629_at	glucocorticoid-induced leucine zipper	GILZ	AI635895	4.8061	Above
39	39721_at	ephrin-B1	EFNB1	U09303	4.7968	Above
40	2094_s_at	v-fos FBJ murine osteosarcoma viral oncogene homolog	FOS	K00650	4.7446	Above

*MLL*
1	307_at	arachidonate 5-lipoxygenase	ALOX5	J03600	-16.8244	Below
2	37280_at	MAD mothers against decapentaplegic Drosophila homolog 1	MADH1	U59912	-15.4460	Below
3	1520_s_at	interleukin 1 beta	IL1B	X04500	-13.6764	Below
4	36908_at	Human macrophage mannose receptor (MRC1) gene, exon 30.	MRC1	M93221	-11.8629	Below
5	33412_at	LGALS1 Lectin, galactoside-binding, soluble, 1 (galectin 1)	LGALS1	AI535946	11.0223	Above
6	2062_at	insulin-like growth factor binding protein 7	IGFBP7	L19182	10.4318	Above
7	35940_at	POU domain class 4 transcription factor 1	POU4F1	X64624	-10.1815	Below
8	39721_at	ephrin-B1	EFNB1	U09303	-9.6158	Below
9	39402_at	interleukin 1 beta	IL1B	M15330	-9.5998	Below
10	1737_s_at	insulin-like growth factor-binding protein 4	IGFBP4	M62403	-9.4119	Below
11	37413_at	dipeptidase 1 renal	DPEP1	J05257	-9.4101	Below
12	40519_at	protein tyrosine phosphatase receptor type C	PTPRC	Y00638	9.3163	Above
13	1971_g_at	fragile histidine triad gene	FHIT	U46922	-9.2257	Below
14	1983_at	cyclin D2	CCND2	X68452	-9.2213	Below
15	38869_at	KIAA1069 protein	KIAA1069	AB028992	-9.1951	Below
16	40520_g_at	protein tyrosine phosphatase receptor type C	PTPRC	Y00638	9.1099	Above
17	1718_at	actin related protein 2/3 complex subunit 2 34 kD	ARPC2	U50523	9.0435	Above
18	34237_at	HBS1 S. cerevisiae like	HBS1L	AB028961	-8.8208	Below
19	1726_at	DNA polymerase, epsilon, catalytic subunit	�	HG919-HT919	-8.4664	Below
20	36643_at	discoidin domain receptor family member 1	DDR1	L20817	-8.4627	Below
21	1325_at	MAD mothers against decapentaplegic Drosophila homolog 1	MADH1	U59423	-8.3762	Below
22	39379_at	Homo sapiens mRNA cDNA DKFZp586C1019	�	AL049397	8.2974	Above
23	36536_at	schwannomin interacting protein 1	SCHIP-1	AF070614	-8.1177	Below
24	564_at	guanine nucleotide binding protein G protein alpha 11 Gq class	GNA11	M69013	-8.1107	Below
25	39705_at	KIAA0700 protein	KIAA0700	AB014600	-7.9334	Below
26	36105_at	Human nonspecific crossreacting antigen mRNA, complete cds.	NCA	M18728	-7.6911	Below
27	174_s_at	intersectin 2	ITSN2	U61167	7.5752	Above
28	39114_at	decidual protein induced by progesterone	DEPP	AB022718	-7.4767	Below
29	40436_g_at	solute carrier family 25 mitochondrial carrier adenine nucleotide translocator member 6	SLC25A6	J03592	7.3952	Above
30	794_at	protein tyrosine phosphatase non-receptor type 6	PTPN6	X62055	7.2192	Above
31	38032_at	KIAA0736 gene product	KIAA0736	AB018279	-7.0718	Below
32	40518_at	protein tyrosine phosphatase receptor type C	PTPRC	Y00062	6.9829	Above
33	41762_at	TIA1 cytotoxic granule-associated RNA-binding protein-like 1	TIAL1	D64015	-6.9118	Below
34	1389_at	membrane metallo-endopeptidase neutral endopeptidase enkephalinase CALLA CD10	MME	J03779	-6.7734	Below
35	39967_at	leucine zipper down-regulated in cancer 1	LDOC1	AB019527	-6.7415	Below
36	188_at	ephrin-B1	EFNB1	U09303	-6.5964	Below
37	160033_s_at	X-ray repair complementing defective repair in Chinese hamster cells 1	XRCC1	NM_006297	-6.5936	Below
38	40913_at	ATPase Ca transporting plasma membrane 4	ATP2B4	W28589	-6.5774	Below
39	37398_at	platelet/endothelial cell adhesion molecule CD31 antigen	PECAM1	AA100961	-6.5675	Below
40	1488_at	protein tyrosine phosphatase receptor type K	PTPRK	L77886	-6.5584	Below

*Novel*
1	41734_at	KIAA0870 protein	KIAA0870	AB020677	-40.5168	Below
2	31892_at	protein tyrosine phosphatase receptor type M	PTPRM	X58288	33.4654	Above
3	995_g_at	protein tyrosine phosphatase receptor type M	PTPRM	X58288	24.7557	Above
4	34676_at	KIAA1099 protein	KIAA1099	AB029022	14.0491	Above
5	37908_at	guanine nucleotide binding protein 11	GNG11	U31384	11.4548	Above
6	37960_at	carbohydrate chondroitin 6/keratan sulfotransferase 2	CHST2	AB014679	10.9971	Above
7	33410_at	integrin alpha 6	ITGA6	S66213	10.0370	Above
8	40585_at	adenylate cyclase 7	ADCY7	D25538	-9.5897	Below
9	33284_at	myeloperoxidase	MPO	M19507	-9.4724	Below
10	41159_at	clathrin heavy polypeptide Hc	CLTC	D21260	9.4489	Above
11	36591_at	tubulin alpha 1 testis specific	TUBA1	X06956	-9.1387	Below
12	37712_g_at	MADS box transcription enhancer factor 2 polypeptide C myocyte enhancer factor 2C	MEF2C	S57212	-9.1225	Below
13	38576_at	H2B histone family member B	H2BFB	AJ223353	-9.0869	Below
14	38408_at	transmembrane 4 superfamily member 2	TM4SF2	L10373	-8.7026	Below
15	33907_at	eukaryotic translation initiation factor 4 gamma 3	EIF4G3	AF012072	-8.3540	Below
16	41273_at	FK506 binding protein 12-rapamycin associated protein 1	FRAP1	AL046940	-8.3212	Below
17	402_s_at	intercellular adhesion molecule 3	ICAM3	X69819	-7.9741	Below
18	35112_at	regulator of G-protein signalling 9	RGS9	AF071476	7.8348	Above
19	34850_at	ubiquitin-conjugating enzyme E2E 3 homologous to yeast UBC4/5	UBE2E3	AB017644	7.8197	Above
20	37030_at	KIAA0887 protein	KIAA0887	AB020694	-7.6343	Below
21	36322_at	fucosyltransferase 7 alpha 1 3 fucosyltransferase	FUT7	AB012668	-7.6240	Below
22	39509_at	Homo sapiens cDNA FLJ22071	�	AI692348	-7.6232	Below
23	40091_at	B-cell CLL/lymphoma 6 zinc finger protein 51	BCL6	U00115	-7.6171	Below
24	37280_at	MAD mothers against decapentaplegic Drosophila homolog 1	MADH1	U59912	7.5991	Above
25	1325_at	MAD mothers against decapentaplegic Drosophila homolog 1	MADH1	U59423	7.5824	Above
26	831_at	DEAD/H Asp-Glu-Ala-Asp/His box polypeptide 10 RNA helicase	DDX10	U28042	7.4276	Above
27	37600_at	extracellular matrix protein 1	ECM1	U68186	-7.2991	Below
28	41266_at	integrin alpha 6	ITGA6	X53586	7.2985	Above
29	36958_at	zyxin	ZYX	X95735	-7.2889	Below
30	36564_at	Human DNA sequence from clone RP5-1174N9 on chromosome 1p34.1-35.3	�	W27419	-7.2848	Below
31	32174_at	solute carrier family 9 sodium/hydrogen exchanger isoform 3 regulatory factor 1	SLC9A3R1	AF015926	-7.2749	Below
32	619_s_at	membrane-spanning 4-domains subfamily A member 2 Fc fragment of IgE high affinity I receptor for beta polypeptide	MS4A2	M27394	-7.2325	Below
33	40749_at	membrane-spanning 4-domains subfamily A member 2 Fc fragment of IgE high affinity I receptor for beta polypeptide	MS4A2	X07203	-7.2063	Below
34	31894_at	centromere protein C 1	CENPC1	M95724	6.9679	Above
35	32319_at	tumor necrosis factor ligand superfamily member 4 tax-transcriptionally activated glycoprotein 1 34kD	TNFSF4	AL022310	6.8225	Above
36	38259_at	syntaxin binding protein 2	STXBP2	AB002559	-6.6992	Below
37	35629_at	hypothetical protein	DJ1042K10.2	AL022238	-6.6968	Below
38	38700_at	cysteine and glycine-rich protein 1	CSRP1	M33146	-6.6962	Below
39	37397_at	Homo sapiens platelet/endothelial cell adhesion molecule-1 (PECAM-1) gene, exon 16 and complete cds.	PECAM	L34657	-6.6934	Below
40	41127_at	solute carrier family 1 glutamate/neutral amino acid transporter member 4	SLC1A4	L14595	-6.6892	Below

*T -ALL*
1	38242_at	B cell linker protein	SLP65	AF068180	-115.8362	Below
2	38319_at	CD3D antigen delta polypeptide TiT3 complex	CD3D	AA919102	27.6995	Above
3	37988_at	CD79B antigen immunoglobulin-associated beta	CD79B	M89957	-23.7294	Below
4	38147_at	SH2 domain protein 1A Duncan s disease lymphoproliferative syndrome	SH2D1A	AL023657	22.4501	Above
5	38522_s_at	CD22 antigen	CD22	X52785	-21.2795	Below
6	35350_at	B cell RAG associated protein	BRAG	AB011170	-19.1460	Below
7	36277_at	Human membran protein (CD3-epsilon) gene, exon 9.	CD3E	M23323	19.0859	Above
8	38604_at	neuropeptide Y	NPY	AI198311	-18.8194	Below
9	33705_at	phosphodiesterase 4B cAMP-specific dunce Drosophila homolog phosphodiesterase E4	PDE4B	L20971	-18.6383	Below
10	36878_f_at	major histocompatibility complex class II DQ beta 1	HLA-DQB1	M60028	-18.5620	Below
11	36638_at	connective tissue growth factor	CTGF	X78947	-18.2772	Below
12	32794_g_at	T cell receptor beta locus	TRB	X00437	17.9081	Above
13	32174_at	solute carrier family 9 sodium/hydrogen exchanger isoform 3 regulatory factor 1	SLC9A3R1	AF015926	17.4427	Above
14	160041_at	protein tyrosine phosphatase non-receptor type 18 brain-derived	PTPN18	X79568	-17.3412	Below
15	38521_at	CD22 antigen	CD22	X59350	-17.0388	Below
16	38018_g_at	CD79A antigen immunoglobulin-associated alpha	CD79A	U05259	-16.7948	Below
17	36571_at	topoisomerase DNA II beta 180kD	TOP2B	X68060	-16.7508	Below
18	1096_g_at	CD19 antigen	CD19	M28170	-16.4583	Below
19	39318_at	T-cell leukemia/lymphoma 1A	TCL1A	X82240	-16.2017	Below
20	41710_at	hypothetical protein	LOC54103	AL079277	-15.9099	Below
21	599_at	H2.0 Drosophila like homeo box 1	HLX1	M60721	-15.5425	Below
22	266_s_at	CD24 antigen small cell lung carcinoma cluster 4 antigen	CD24	L33930	-15.0123	Below
23	36502_at	PFTAIRE protein kinase 1	PFTK1	AB020641	-14.9972	Below
24	39114_at	decidual protein induced by progesterone	DEPP	AB022718	-14.9886	Below
25	37539_at	RalGDS-like gene KIAA0959 protein	KIAA0959	AB023176	-14.6872	Below
26	40775_at	integral membrane protein 2A	ITM2A	AL021786	14.5666	Above
27	34033_s_at	leukocyte immunoglobulin-like receptor subfamily A with TM domain member 2	LILRA2	AF025531	-14.3809	Below
28	2031_s_at	cyclin-dependent kinase inhibitor 1A p21 Cip1	CDKN1A	U03106	-14.1071	Below
29	38051_at	mal T-cell differentiation protein	MAL	X76220	14.0743	Above
30	35794_at	KIAA0942 protein	KIAA0942	AB023159	-13.9659	Below
31	41156_g_at	catenin cadherin-associated protein alpha 1 102kD	CTNNA1	U03100	-13.8135	Below
32	32979_at	GRB2-associated binding protein 1	GAB1	U43885	-13.5842	Below
33	32562_at	endoglin Osler-Rendu-Weber syndrome 1	ENG	X72012	-13.4209	Below
34	36536_at	schwannomin interacting protein 1	SCHIP-1	AF070614	-13.4172	Below
35	36108_at	major histocompatibility complex class II DQ beta 1	HLA-DQB1	M16276	-13.3518	Below
36	41734_at	KIAA0870 protein	KIAA0870	AB020677	-13.2672	Below
37	41153_f_at	Homo sapiens alphaE-catenin (CTNNA1) gene, exon 18 and complete cds.	CTNNA1	AF102803	-12.7927	Below
38	37710_at	MADS box transcription enhancer factor 2 polypeptide C myocyte enhancer factor 2C	MEF2C	L08895	-12.7716	Below
39	39893_at	guanine nucleotide binding protein G protein gamma 7	GNG7	AB010414	-12.7696	Below
40	37908_at	guanine nucleotide binding protein 11	GNG11	U31384	-12.7353	Below

TEL-AML1
1	38578_at	tumor necrosis factor receptor superfamily member 7	TNFRSF7	M63928	15.2209	Above
2	38203_at	potassium intermediate/small conductance calcium-activated channel subfamily N member 1	KCNN1	U69883	15.0804	Above
3	36524_at	Rho guanine nucleotide exchange factor GEF 4	ARHGEF4	AB029035	14.9774	Above
4	37780_at	piccolo presynaptic cytomatrix protein	PCLO	AB011131	14.1405	Above
5	35614_at	transcription factor-like 5 basic helix-loop-helix	TCFL5	AB012124	12.9369	Above
6	160029_at	protein kinase C beta 1	PRKCB1	X07109	12.5429	Above
7	1980_s_at	non-metastatic cells 2 protein NM23B expressed in	NME2	X58965	-12.5035	Below
8	1488_at	protein tyrosine phosphatase receptor type K	PTPRK	L77886	12.3871	Above
9	34194_at	Homo sapiens cDNA FLJ21697	�	AL049313	12.1089	Above
10	37908_at	guanine nucleotide binding protein 11	GNG11	U31384	11.4322	Above
11	40272_at	collapsin response mediator protein 1	CRMP1	D78012	11.0625	Above
12	41097_at	telomeric repeat binding factor 2	TERF2	AF002999	11.0133	Above
13	33690_at	Homo sapiens mRNA cDNA DKFZp434A202	�	AL080190	10.8763	Above
14	32730_at	Homo sapiens mRNA for KIAA1750	�	AL080059	10.7439	Above
15	1325_at	MAD mothers against decapentaplegic Drosophila homolog 1	MADH1	U59423	10.5332	Above
16	41819_at	FYN-binding protein FYB-120/130	FYB	U93049	10.3692	Above
17	1299_at	telomeric repeat binding factor 2	TERF2	X93512	10.2921	Above
18	35665_at	phosphoinositide-3-kinase class 3	PIK3C3	Z46973	10.0568	Above
19	36537_at	Rho-specific guanine nucleotide exchange factor p114	P114-RHO-GEF	AB011093	9.8824	Above
20	37280_at	MAD mothers against decapentaplegic Drosophila homolog 1	MADH1	U59912	9.8662	Above
21	1936_s_at	proto-oncogene c-myc, alt. transcript 3, ORF 114	�	HG3523-HT4899	-9.6621	Below
22	1077_at	recombination activating gene 1	RAG1	M29474	9.4563	Above
23	38763_at	Human (clone D21-1) L-iditol-2 dehydrogenase gene, exon 9 and complete cds.	�	L29254	-9.2719	Below
24	41295_at	GTT1 protein	GTT1	AL041780	-9.1813	Below
25	36008_at	protein tyrosine phosphatase type IVA member 3	PTP4A3	AF041434	9.1682	Above
26	38570_at	major histocompatibility complex class II DO beta	HLA-DOB	X03066	9.0394	Above
27	32163_f_at	EST	�	AA216639	9.0392	Above
28	40570_at	forkhead box O1A rhabdomyosarcoma	FOXO1A	AF032885	8.9931	Above
29	32724_at	phytanoyl-CoA hydroxylase Refsum disease	PHYH	AF023462	8.9571	Above
30	932_i_at	zinc finger protein 91 HPF7 HTF10	ZNF91	L11672	8.8075	Above
31	37343_at	inositol 1 4 5-triphosphate receptor type 3	ITPR3	U01062	8.7321	Above
32	33447_at	myosin light polypeptide regulatory non-sarcomeric 20kD	MLCB	X54304	-8.6848	Below
33	35362_at	myosin X	MYO10	AB018342	8.6700	Above
34	38906_at	spectrin alpha erythrocytic 1 elliptocytosis 2	SPTA1	M61877	8.5010	Above
35	324_f_at	basic transcription factor 3	BTF3	HG1515-HT1515	-8.4705	Below
36	39329_at	actinin alpha 1	ACTN1	X15804	-8.3219	Below
37	577_at	midkine neurite growth-promoting factor 2	MDK	M94250	8.2693	Above
38	40729_s_at	nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1	NFKBIL1	Y14768	8.2000	Above
39	41442_at	core-binding factor runt domain alpha subunit 2 translocated to 3	CBFA2T3	AB010419	8.0604	Above
40	36275_at	Homo sapiens mRNA from chromosome 5q21-22 clone FBR89	�	AB002438	7.8550	Above

Illustrated below are the results of a two-dimensional hierarchical clustering algorithm of the 327 diagnostic ALL cases using the top 280 probe sets selected by the T-statistics method. Some probe sets were chosen for more than one diagnostic group, and thus there are only 248 unique probe sets represented.

Figure 16. Hierarchical cluster of 327 Diagnostic ALL samples with genes selected by the T-statistics method.

Wilkins'
This method of selecting genes uses the weighted sum of three components to estimate the discriminative value of each gene. The higher the score, the better the gene is at discriminating between the two classes. The input to the scoring method is preprocessed and normalized data. The idea of the metric is that a gene is a good discriminator if (1) it is expressed in one class and not in the other, or if the gene is expressed in both classes, but significantly more so in one than the other, or (2) the gene is present in most samples, and the data are pure, in the sense that there is a threshold expression value for the gene where the gene generally has expression levels larger than the threshold in one class, and smaller than the threshold in the other class. The components of the metric were quantified as follows. For a gene, assume PR₁ is the ratio of "present" samples to all samples in class 1, where present means that the gene's expression value was not preprocessed to a constant (1). Assume PR₂ is defined similarly for class 2. The first component of the metric, M₁, is estimated as the absolute difference between PR₁ and PR₂. This value is between 0 (when the gene is equally present in both classes) and 1 (when the gene is expressed in one class and not in the other). The second component of the metric, M₂, measures the extent to which the gene is present overall, and is defined as the average of PR₁ and PR₂. The final component, M₃, estimates the "purity", or existence of a threshold value. The gene expression values for the present samples are sorted into ascending order and a vector of their class labels is built, for example {+, +, +, -, -, -, +, -, -, +, -}. The next step is to find the best place to partition the samples so that the expression values for one class (maybe +) are less than the partition point, and the values from the other class are larger. Let L_C1 and L_C2 be the number of class 1 and class 2 samples on the left side of the partition, respectively. Assume R₁ and R_C2 are defined similarly for the right side of the partition. Then the purity is estimated as: max {L_C1 - L_C2 + R_C2 - R_C1, L_C2 - L_C1 + R_C1 - R_C2} / number of total present samples. Each possible partition is checked. In the example above, the partition {+, +, +, || -, -, -, +, -, -, +, -} is the best partition, with a purity value of M₃ = 7 / 11 = 0.64. The score for the gene is the weighted sum of 0.5*M₁ + 0.25*M₂ + 0.25*M₃. The top 50 genes for each subgroup selected by this metric are listed in Table 14. For class prediction all 50 genes were used, unless otherwise stated.

Table 14. Genes Selected by Wilkins'


	Affymetrix number	Gene Name	Gene Symbol	Reference number	Train set score	Above/ Below Mean
*BCR-ABL*
1	32319_at	tumor necrosis factor ligand superfamily member 4 tax-transcriptionally activated glycoprotein 1 34kD	TNFSF4	AL022310	0.6354	Above
2	37479_at	CD72 antigen	CD72	M54992	0.6352	Below
3	1211_s_at	CASP2 and RIPK1 domain containing adaptor with death domain	CRADD	U84388	0.6265	Above
4	37397_at	platelet/endothelial cell adhesion molecule-1 (PECAM-1) gene	PECAM	L34657	0.6161	Above
5	33162_at	insulin receptor	INSR	X02160	0.6118	Below
6	39691_at	SH3-containing protein SH3GLB1	SH3GLB1	AB007960	0.6089	Above
7	1558_g_at	p21/Cdc42/Rac1-activated kinase 1 yeast Ste20-related	PAK1	U24152	0.6087	Above
8	34759_at	Human hbc647 mRNA sequence	�	U68494	0.6061	Above
9	33774_at	caspase 8 apoptosis-related cysteine protease	CASP8	X98172	0.6040	Above
10	1326_at	caspase 10 apoptosis-related cysteine protease	CASP10	U60519	0.6021	Above
11	38312_at	DKFZp564O222 from clone DKFZp564O222	�	AL050002	0.6010	Above
12	35970_g_at	M-phase phosphoprotein 9	MPHOSPH9	N23137	0.5989	Above
13	41273_at	FK506 binding protein 12-rapamycin associated protein 1	FRAP1	AL046940	0.5989	Above
14	40798_s_at	a disintegrin and metalloproteinase domain 10	ADAM10	Z48579	0.5980	Above
15	40953_at	calponin 3 acidic	CNN3	S80562	0.5972	Above
16	1434_at	phosphatase and tensin homolog mutated in multiple advanced cancers 1	PTEN	U92436	0.5963	Below
17	38966_at	glycoprotein synaptic 2	GPSN2	AF038958	0.5953	Above
18	35991_at	Sm protein F	LSM6	AA917945	0.5938	Above
19	330_s_at	tubulin, alpha 1, isoform 44	TUBA1	HG2259-HT2348	0.5938	Above
20	38032_at	KIAA0736 gene product	KIAA0736	AB018279	0.5934	Above
21	1983_at	cyclin D2	CCND2	X68452	0.5927	Above
22	36194_at	low density lipoprotein-related protein-associated protein 1 alpha-2-macroglobulin receptor-associated protein 1	LRPAP1	M63959	0.5914	Below
23	34460_at	peripheral benzodiazepine receptor-associated protein 1	PRAX-1	AB014512	0.5911	Above
24	2001_g_at	ataxia telangiectasia mutated includes complementation groups A C and D	ATM	U26455	0.5910	Above
25	31443_at	AML1	AML1	S76346	0.5896	Above
26	33410_at	integrin alpha 6	ITGA6	S66213	0.5896	Above
27	37472_at	mannosidase beta A lysosomal	MANBA	U60337	0.5887	Below
28	36099_at	splicing factor arginine/serine-rich 1 splicing factor 2 alternate splicing factor	SFRS1	M69040	0.5877	Below
29	38636_at	immunoglobulin superfamily containing leucine-rich repeat	ISLR	AB003184	0.5858	Above
30	34314_at	ribonucleotide reductase M1 polypeptide	RRM1	X59543	0.5858	Below
31	36129_at	KIAA0397 gene product	KIAA0397	AB007857	0.5858	Above
32	40264_g_at	zinc finger protein-like 1	ZFPL1	AF001891	0.5858	Above
33	37399_at	aldo-keto reductase family 1 member C3 3-alpha hydroxysteroid dehydrogenase type II	AKR1C3	D17793	0.5852	Above
34	38160_at	lymphocyte antigen 75	LY75	AF011333	0.5832	Above
35	41649_at	FOXJ2 forkhead factor	LOC55810	AF038177	0.5832	Above
36	36591_at	tubulin alpha 1 testis specific	TUBA1	X06956	0.5832	Above
37	40167_s_at	CS box-containing WD protein	LOC55884	AF038187	0.5832	Above
38	2064_g_at	excision repair cross-complementing rodent repair deficiency complementation group	ERCC5	L20046	0.5832	Above
39	39729_at	Human natural killer cell enhancing factor (NKEFB) mRNA, complete cds.	NKEFB	L19185	0.5829	Below
40	38270_at	poly ADP-ribose glycohydrolase	PARG	AF005043	0.5828	Below
41	40613_at	uncharacterized hypothalamus protein HT012	HT012	AL031775	0.5819	Below
42	39070_at	singed Drosophila like sea urchin fascin homolog like	SNL	U03057	0.5813	Above
43	40782_at	short-chain dehydrogenase/reductase 1	SDR1	AF061741	0.5813	Above
44	34256_at	sialyltransferase 9 CMP-NeuAc lactosylceramide alpha-2 3-sialyltransferase GM3 synthase	SIAT9	AB018356	0.5797	Above
45	41836_at	protein with polyglutamine repeat calcium ca2 homeostasis endoplasmic reticulum protein	ERPROT213-21	U94836	0.5777	Above
46	35681_r_at	zinc finger homeobox 1B	ZFHX1B	AB011141	0.5759	Below
47	37190_at	WAS protein family member 1	WASF1	D87459	0.5759	Below
48	32788_at	RAN binding protein 2	RANBP2	D42063	0.5756	Above
49	828_at	prostaglandin E receptor 2 subtype EP2 53kD	PTGER2	U19487	0.5740	Above
50	38220_at	dihydropyrimidine dehydrogenase	DPYD	U20938	0.5737	Above

*E2A-PBX1*
1	32063_at	pre-B-cell leukemia transcription factor 1	PBX1	M86546	0.8750	Above
2	38994_at	STAT induced STAT inhibitor-2	STATI2	AF037989	0.8252	Below
3	33355_at	Homo sapiens cDNA FLJ12900 fis clone NT2RP2004321 (by CELERA serach of target sequence = PBX1)	PBX1	AL049381	0.8040	Above
4	40454_at	FAT tumor suppressor Drosophila homolog	FAT	X87241	0.7899	Above
5	753_at	nidogen 2	NID2	D86425	0.7368	Above
6	717_at	GS3955 protein	GS3955	D87119	0.7306	Above
7	1786_at	c-mer proto-oncogene tyrosine kinase	MERTK	U08023	0.7300	Above
8	39070_at	singed Drosophila like sea urchin fascin homolog like	SNL	U03057	0.7271	Below
9	1065_at	fms-related tyrosine kinase 3	FLT3	U02687	0.7160	Below
10	36650_at	cyclin D2	CCND2	D13639	0.7151	Below
11	33513_at	signaling lymphocytic activation molecule	SLAM	U33017	0.7096	Above
12	33748_at	minor histocompatibility antigen HA-1	KIAA0223	D86976	0.7084	Below
13	37225_at	KIAA0172 protein	KIAA0172	D79994	0.7033	Above
14	38717_at	DKFZP586A0522 protein	DKFZP586A0522	AL050159	0.7003	Below
15	854_at	B lymphoid tyrosine kinase	BLK	S76617	0.6982	Above
16	33641_g_at	nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1	NFKBIL1	Y14768	0.6975	Below
17	40468_at	KIAA0554 protein	KIAA0554	AB011126	0.6971	Below
18	41266_at	integrin alpha 6	ITGA6	X53586	0.6965	Below
19	36536_at	schwannomin interacting protein 1	SCHIP-1	AF070614	0.6938	Below
20	362_at	protein kinase C zeta	PRKCZ	Z15108	0.6904	Above
21	755_at	inositol 1 4 5-triphosphate receptor type 1	ITPR1	D26070	0.6877	Below
22	307_at	arachidonate 5-lipoxygenase	ALOX5	J03600	0.6875	Below
23	39614_at	KIAA0802 protein	KIAA0802	AB018345	0.6863	Above
24	1563_s_at	tumor necrosis factor receptor superfamily member 1A	TNFRSF1A	M58286	0.6837	Below
25	38748_at	adenosine deaminase RNA-specific B1 homolog of rat RED1	ADARB1	U76421	0.6763	Above
26	41409_at	basement membrane-induced gene	ICB-1	AF044896	0.6757	Below
27	34892_at	tumor necrosis factor receptor superfamily member 10b	TNFRSF10B	AF016266	0.6726	Below
28	40648_at	c-mer proto-oncogene tyrosine kinase	MERTK	U08023	0.6710	Above
29	38408_at	transmembrane 4 superfamily member 2	TM4SF2	L10373	0.6667	Below
30	34583_at	fms-related tyrosine kinase 3	FLT3	U02687	0.6665	Below
31	36900_at	stromal interaction molecule 1	STIM1	U52426	0.6650	Below
32	37625_at	interferon regulatory factor 4	IRF4	U52682	0.6636	Above
33	38340_at	huntingtin interacting protein-1-related	KIAA0655	AB014555	0.6609	Above
34	1830_s_at	transforming growth factor beta 1	TGFB1	M38449	0.6608	Below
35	37099_at	arachidonate 5-lipoxygenase-activating protein	ALOX5AP	AI806222	0.6605	Below
36	38254_at	KIAA0882 protein	KIAA0882	AB020689	0.6539	Below
37	37641_at	Human gene for hepatitis C-associated microtubular aggregate protein p44, exon 9 and complete cds.	�	D28915	0.6531	Below
38	33865_at	adenovirus 5 E1A binding protein	BS69	AA127624	0.6515	Below
39	40729_s_at	nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1	NFKBIL1	Y14768	0.6502	Below
40	40113_at	GS3955 protein	GS3955	D87119	0.6476	Above
41	32979_at	GRB2-associated binding protein 1	GAB1	U43885	0.6457	Below
42	36591_at	tubulin alpha 1 testis specific	TUBA1	X06956	0.6427	Below
43	38739_at	v-ets avian erythroblastosis virus E26 oncogene homolog 2	ETS2	AF017257	0.6424	Below
44	37485_at	fatty-acid-Coenzyme A ligase very long-chain 1	FACVL1	D88308	0.6363	Above
45	538_at	CD34 antigen	CD34	S53911	0.6326	Below
46	37893_at	protein tyrosine phosphatase non-receptor type 2	PTPN2	AI828880	0.6318	Above
47	41017_at	myosin-binding protein H	MYBPH	U27266	0.6297	Above
48	37967_at	lymphocyte antigen 117	LY117	AF000424	0.6260	Below
49	37281_at	KIAA0233 gene product	KIAA0233	D87071	0.6250	Below
50	35675_at	vinexin beta SH3-containing adaptor molecule-1	SCAM-1	AF037261	0.6229	Below

Hyperdiploid > 50
1	39878_at	protocadherin 9	PCDH9	AI524125	0.5838	Below
2	41470_at	prominin mouse like 1	PROML1	AF027208	0.5616	Above
3	39069_at	AE-binding protein 1	AEBP1	AF053944	0.5423	Below
4	1520_s_at	interleukin 1 beta	IL1B	X04500	0.5399	Above
5	578_at	Human recombination acitivating protein (RAG2) gene, last exon	RAG2	M94633	0.5208	Below
6	32251_at	hypothetical protein FLJ21174	FLJ21174	AA149307	0.5164	Above
7	40480_s_at	FYN oncogene related to SRC FGR YES	FYN	M14333	0.5090	Above
8	38604_at	neuropeptide Y	NPY	AI198311	0.5083	Above
9	40903_at	ATPase H transporting lysosomal vacuolar proton pump membrane sector associated protein M8-9	APT6M8-9	AL049929	0.5080	Above
10	38968_at	SH3-domain binding protein 5 BTK-associated	SH3BP5	AB005047	0.5057	Above
11	37272_at	inositol 1 4 5-trisphosphate 3-kinase B	ITPKB	X57206	0.5025	Below
12	35688_g_at	mature T-cell proliferation 1	MTCP1	Z24459	0.5018	Above
13	1488_at	protein tyrosine phosphatase receptor type K	PTPRK	L77886	0.4977	Below
14	36885_at	spleen tyrosine kinase	SYK	L28824	0.4964	Below
15	1630_s_at	tyrosine kinase syk	syk	HG3730-HT4000	0.4913	Below
16	38317_at	transcription elongation factor A SII like 1	TCEAL1	M99701	0.4901	Above
17	38649_at	KIAA0970 protein	KIAA0970	AB023187	0.4898	Below
18	39721_at	ephrin-B1	EFNB1	U09303	0.4895	Above
19	33307_at	kraken-like	BK126B4.1	AL022316	0.4880	Below
20	38518_at	sex comb on midleg Drosophila like 2	SCML2	Y18004	0.4879	Above
21	39402_at	interleukin 1 beta	IL1B	M15330	0.4750	Above
22	36489_at	phosphoribosyl pyrophosphate synthetase 1	PRPS1	D00860	0.4718	Above
23	37747_at	Human annexin V (ANX5) gene, exon 13.	(ANX5	U05770	0.4717	Above
24	40200_at	heat shock transcription factor 1	HSF1	M64673	0.4689	Below
25	35940_at	POU domain class 4 transcription factor 1	POU4F1	X64624	0.4685	Above
26	35727_at	hypothetical protein FLJ20517	FLJ20517	AI249721	0.4675	Below
27	1357_at	ubiquitin specific protease 4 proto-oncogene	USP4	U20657	0.4670	Below
28	36592_at	prohibitin	PHB	S85655	0.4668	Above
29	37014_at	myxovirus influenza resistance 1 homolog of murine interferon-inducible protein p78	MX1	M33882	0.4635	Above
30	40891_f_at	DNA segment on chromosome X unique 9879 expressed sequence	DXS9879E	X92896	0.4608	Above
31	40846_g_at	interleukin enhancer binding factor 3 90kD	ILF3	U10324	0.4605	Below
32	41132_r_at	heterogeneous nuclear ribonucleoprotein H2 H	HNRPH2	U01923	0.4605	Above
33	37280_at	MAD mothers against decapentaplegic Drosophila homolog 1	MADH1	U59912	0.4595	Below
34	35939_s_at	POU domain class 4 transcription factor 1	POU4F1	L20433	0.4594	Above
35	890_at	ubiquitin-conjugating enzyme E2A RAD6 homolog	UBE2A	M74524	0.4570	Above
36	38738_at	SMT3 suppressor of mif two 3 yeast homolog 1	SMT3H1	X99584	0.4568	Above
37	38458_at	Human cytochrome b5 (CYB5) gene, exon 6 and complete cds.	CYB5	L39945	0.4552	Above
38	38869_at	KIAA1069 protein	KIAA1069	AB028992	0.4549	Above
39	915_at	interferon-induced protein with tetratricopeptide repeats 1	IFIT1	M24594	0.4544	Above
40	38408_at	transmembrane 4 superfamily member 2	TM4SF2	L10373	0.4535	Above
41	39301_at	calpain 3 p94	CAPN3	X85030	0.4533	Below
42	41425_at	Friend leukemia virus integration 1	FLI1	M98833	0.4519	Below
43	2094_s_at	v-fos FBJ murine osteosarcoma viral oncogene homolog	FOS	K00650	0.4514	Above
44	36605_at	transcription factor 4	TCF4	M74719	0.4497	Above
45	37709_at	DNA segment numerous copies expressed probes GS1 gene	DXF68S1E	M86934	0.4493	Above
46	36128_at	transmembrane trafficking protein	TMP21	L40397	0.4488	Above
47	171_at	von Hippel-Lindau binding protein 1	VBP1	U56833	0.4473	Above
48	41490_at	phosphoribosyl pyrophosphate synthetase 2	PRPS2	Y00971	0.4466	Above
49	36536_at	schwannomin interacting protein 1	SCHIP-1	AF070614	0.4448	Above
50	35843_at	Homo sapiens mRNA cDNA DKFZp434D0935		L40402	0.4443	Above

*MLL*
1	39402_at	interleukin 1 beta	IL1B	M15330	0.7355	Below
2	307_at	arachidonate 5-lipoxygenase	ALOX5	J03600	0.7221	Below
3	1389_at	membrane metallo-endopeptidase neutral endopeptidase enkephalinase CALLA CD10	MME	J03779	0.7178	Below
4	37280_at	MAD mothers against decapentaplegic Drosophila homolog 1	MADH1	U59912	0.7021	Below
5	36650_at	cyclin D2	CCND2	D13639	0.6759	Below
6	37043_at	inhibitor of DNA binding 3 dominant negative helix-loop-helix protein	ID3	AL021154	0.6743	Below
7	1520_s_at	interleukin 1 beta	IL1B	X04500	0.6689	Below
8	40913_at	ATPase Ca transporting plasma membrane 4	ATP2B4	W28589	0.6684	Below
9	36536_at	schwannomin interacting protein 1	SCHIP-1	AF070614	0.6554	Below
10	37398_at	platelet/endothelial cell adhesion molecule CD31 antigen	PECAM1	AA100961	0.6548	Below
11	39114_at	decidual protein induced by progesterone	DEPP	AB022718	0.6478	Below
12	37967_at	lymphocyte antigen 117	LY117	AF000424	0.6432	Below
13	1325_at	MAD mothers against decapentaplegic Drosophila homolog 1	MADH1	U59423	0.6421	Below
14	38336_at	KIAA1013 protein	KIAA1013	AB023230	0.6395	Below
15	577_at	midkine neurite growth-promoting factor 2	MDK	M94250	0.6363	Below
16	38671_at	KIAA0620 protein	KIAA0620	AB014520	0.6353	Below
17	33412_at	LGALS1 Lectin, galactoside-binding, soluble, 1	LGALS1	AI535946	0.6351	Above
18	40451_at	hypothetical protein FLJ21434	FLJ21434	AL080203	0.6350	Below
19	36908_at	Human macrophage mannose receptor (MRC1) gene, exon 30.	MRC1	M93221	0.6290	Below
20	963_at	ligase IV DNA ATP-dependent	LIG4	X83441	0.6282	Below
21	41346_at	like-glycosyltransferase	LARGE	AJ007583	0.6214	Below
22	32207_at	membrane protein palmitoylated 1 55kD	MPP1	M64925	0.6155	Below
23	2062_at	insulin-like growth factor binding protein 7	IGFBP7	L19182	0.6145	Above
24	38408_at	transmembrane 4 superfamily member 2	TM4SF2	L10373	0.6137	Below
25	854_at	B lymphoid tyrosine kinase	BLK	S76617	0.6075	Above
26	32193_at	plexin C1	PLXNC1	AF030339	0.6065	Above
27	35939_s_at	POU domain class 4 transcription factor 1	POU4F1	L20433	0.6046	Below
28	33705_at	phosphodiesterase 4B cAMP-specific dunce Drosophila homolog phosphodiesterase E4	PDE4B	L20971	0.5991	Below
29	34168_at	deoxynucleotidyltransferase terminal	DNTT	M11722	0.5979	Below
30	36383_at	v-ets avian erythroblastosis virus E26 oncogene related	ERG	M17254	0.5976	Below
31	38968_at	SH3-domain binding protein 5 BTK-associated	SH3BP5	AB005047	0.5976	Below
32	39263_at	2 5 oligoadenylate synthetase 2	OAS2	M87434	0.5967	Below
33	39329_at	actinin alpha 1	ACTN1	X15804	0.5953	Below
34	34699_at	CD2-associated protein	CD2AP	AL050105	0.5945	Below
35	1267_at	protein kinase C eta	PRKCH	M55284	0.5941	Below
36	35172_at	tyrosylprotein sulfotransferase 2	TPST2	AF049891	0.5937	Below
37	38124_at	midkine neurite growth-promoting factor 2	MDK	X55110	0.5936	Below
38	33813_at	tumor necrosis factor receptor superfamily member 1B	TNFRSF1B	AI813532	0.5934	Below
39	34176_at	hypothetical protein from clone 643	LOC57228	AF091087	0.5930	Below
40	39424_at	tumor necrosis factor receptor superfamily member 14 herpesvirus entry mediator	TNFRSF14	U70321	0.5930	Below
41	40729_s_at	nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1	NFKBIL1	Y14768	0.5905	Below
42	32607_at	brain acid-soluble protein 1	BASP1	AF039656	0.5905	Above
43	38342_at	KIAA0239 protein	KIAA0239	D87076	0.5896	Below
44	32533_s_at	vesicle-associated membrane protein 5 myobrevin	VAMP5	AF054825	0.5880	Below
45	39330_s_at	actinin alpha 1	ACTN1	M95178	0.5867	Below
46	40519_at	protein tyrosine phosphatase receptor type C	PTPRC	Y00638	0.5848	Above
47	39338_at	S100 calcium-binding protein A10 annexin II ligand calpactin I light polypeptide p11	S100A10	AI201310	0.5844	Above
48	35940_at	POU domain class 4 transcription factor 1	POU4F1	X64624	0.5824	Below
49	39712_at	S100 calcium-binding protein A13	S100A13	AI541308	0.5818	Below
50	39379_at	Homo sapiens mRNA cDNA DKFZp586C1019 from clone DKFZp586C1019	�	AL049397	0.5811	Above

Novel
1	31892_at	protein tyrosine phosphatase receptor type M	PTPRM	X58288	0.8668	Above
2	41734_at	KIAA0870 protein	KIAA0870	AB020677	0.8614	Below
3	995_g_at	protein tyrosine phosphatase receptor type M	PTPRM	X58288	0.8505	Above
4	994_at	protein tyrosine phosphatase receptor type M	PTPRM	X58288	0.7694	Above
5	37967_at	lymphocyte antigen 117	LY117	AF000424	0.7399	Below
6	34676_at	KIAA1099 protein	KIAA1099	AB029022	0.7298	Above
7	41159_at	clathrin heavy polypeptide Hc	CLTC	D21260	0.7283	Above
8	39728_at	interferon gamma-inducible protein 30	IFI30	J03909	0.7138	Below
9	37542_at	lipoma HMGIC fusion partner-like 2	LHFPL2	D86961	0.7069	Above
10	35350_at	B cell RAG associated protein	BRAG	AB011170	0.7049	Below
11	41438_at	KIAA1451 protein	KIAA1451	AL049923	0.6999	Below
12	34370_at	archain 1	ARCN1	X81198	0.6999	Below
13	36029_at	chromosome 11 open reading frame 8	C11ORF8	U57911	0.6964	Above
14	37960_at	carbohydrate chondroitin 6/keratan sulfotransferase 2	CHST2	AB014679	0.6947	Above
15	35869_at	MD-1 RP105-associated	MD-1	AB020499	0.6908	Below
16	36601_at	vinculin	VCL	M33308	0.6908	Below
17	40775_at	integral membrane protein 2A	ITM2A	AL021786	0.6879	Above
18	37281_at	KIAA0233 gene product	KIAA0233	D87071	0.6837	Below
19	957_at	arrestin, beta 2	ARRB2	HG2059-HT2114	0.6744	Below
20	33284_at	myeloperoxidase	MPO	M19507	0.6712	Below
21	40585_at	adenylate cyclase 7	ADCY7	D25538	0.6712	Below
22	37908_at	guanine nucleotide binding protein 11	GNG11	U31384	0.6656	Above
23	40167_s_at	CS box-containing WD protein	LOC55884	AF038187	0.6581	Below
24	38576_at	H2B histone family member B	H2BFB	AJ223353	0.6576	Below
25	36591_at	tubulin alpha 1 testis specific	TUBA1	X06956	0.6576	Below
26	37712_g_at	MADS box transcription enhancer factor 2 polypeptide C myocyte enhancer factor 2C	MEF2C	S57212	0.6576	Below
27	33924_at	KIAA1091 protein	KIAA1091	AB029014	0.6484	Below
28	32724_at	phytanoyl-CoA hydroxylase Refsum disease	PHYH	AF023462	0.6466	Above
29	33358_at	EST (retina)		W29087	0.6457	Above
30	33740_at	chromosome 1 open reading frame 2	C1ORF2	AF023268	0.6441	Below
31	36588_at	KIAA0810 protein	KIAA0810	AB018353	0.6441	Below
32	38802_at	progesterone binding protein	HPR6.6	Y12711	0.6441	Below
33	38408_at	transmembrane 4 superfamily member 2	TM4SF2	L10373	0.6440	Below
34	32227_at	proteoglycan 1 secretory granule	PRG1	X17042	0.6409	Below
35	34840_at	Homo sapiens cDNA FLJ22642 fis clone HSI06970		AI700633	0.6409	Below
36	1131_at	mitogen-activated protein kinase kinase 2	MAP2K2	L11285	0.6409	Below
37	33410_at	integrin alpha 6	ITGA6	S66213	0.6391	Above
38	38006_at	CD48 antigen B-cell membrane protein	CD48	M37766	0.6342	Below
39	33907_at	eukaryotic translation initiation factor 4 gamma 3	EIF4G3	AF012072	0.6304	Below
40	41273_at	FK506 binding protein 12-rapamycin associated protein 1	FRAP1	AL046940	0.6304	Below
41	39781_at	insulin-like growth factor-binding protein 4	IGFBP4	U20982	0.6301	Below
42	39893_at	guanine nucleotide binding protein G protein gamma 7	GNG7	AB010414	0.6301	Below
43	37326_at	proteolipid protein 2 colonic epithelium-enriched	PLP2	U93305	0.6267	Below
44	36687_at	cytochrome c oxidase subunit VIIb	COX7B	N50520	0.6266	Below
45	40423_at	KIAA0903 protein	KIAA0903	AB020710	0.6254	Above
46	32542_at	four and a half LIM domains 1	FHL1	AF063002	0.6236	Below
47	33232_at	cysteine-rich protein 1 intestinal	CRIP1	AI017574	0.6211	Below
48	37280_at	MAD mothers against decapentaplegic Drosophila homolog 1	MADH1	U59912	0.6208	Above
49	1325_at	MAD mothers against decapentaplegic Drosophila homolog 1	MADH1	U59423	0.6208	Above
50	40729_s_at	nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1	NFKBIL1	Y14768	0.6199	Below

T-ALL
1	38242_at	B cell linker protein	SLP65	AF068180	0.8683	Below
2	37988_at	CD79B antigen immunoglobulin-associated beta	CD79B	M89957	0.8422	Below
3	1096_g_at	CD19 antigen	CD19	M28170	0.8181	Below
4	39318_at	T-cell leukemia/lymphoma 1A	TCL1A	X82240	0.8128	Below
5	38018_g_at	CD79A antigen immunoglobulin-associated alpha	CD79A	U05259	0.8127	Below
6	36878_f_at	major histocompatibility complex class II DQ beta 1	HLA-DQB1	M60028	0.8053	Below
7	38147_at	SH2 domain protein 1A Duncan s disease lymphoproliferative syndrome	SH2D1A	AL023657	0.8016	Above
8	35350_at	B cell RAG associated protein	BRAG	AB011170	0.7914	Below
9	38051_at	mal T-cell differentiation protein	MAL	X76220	0.7900	Above
10	266_s_at	CD24 antigen small cell lung carcinoma cluster 4 antigen	CD24	L33930	0.7867	Below
11	38521_at	CD22 antigen	CD22	X59350	0.7856	Below
12	37344_at	major histocompatibility complex class II DM alpha	HLA-DMA	X62744	0.7835	Below
13	34033_s_at	leukocyte immunoglobulin-like receptor subfamily A with TM domain member 2	LILRA2	AF025531	0.7761	Below
14	36638_at	connective tissue growth factor	CTGF	X78947	0.7755	Below
15	38213_at	galactosidase alpha	GLA	U78027	0.7701	Below
16	41734_at	KIAA0870 protein	KIAA0870	AB020677	0.7693	Below
17	37711_at	MADS box transcription enhancer factor 2 polypeptide C myocyte enhancer factor 2C	MEF2C	S57212	0.7560	Below
18	36239_at	POU domain class 2 associating factor 1	POU2AF1	Z49194	0.7440	Below
19	38319_at	CD3D antigen delta polypeptide TiT3 complex	CD3D	AA919102	0.7426	Above
20	38894_g_at	neutrophil cytosolic factor 4 40kD	NCF4	AL008637	0.7422	Below
21	33705_at	phosphodiesterase 4B cAMP-specific dunce Drosophila homolog phosphodiesterase E4	PDE4B	L20971	0.7414	Below
22	38017_at	CD79A antigen immunoglobulin-associated alpha	CD79A	U05259	0.7360	Below
23	41156_g_at	catenin cadherin-associated protein alpha 1 102kD	CTNNA1	U03100	0.7315	Below
24	38994_at	STAT induced STAT inhibitor-2	STATI2	AF037989	0.7292	Below
25	37710_at	MADS box transcription enhancer factor 2 polypeptide C myocyte enhancer factor 2C	MEF2C	L08895	0.7283	Below
26	41155_at	catenin cadherin-associated protein alpha 1 102kD	CTNNA1	U03100	0.7278	Below
27	40570_at	forkhead box O1A rhabdomyosarcoma	FOXO1A	AF032885	0.7258	Below
28	34224_at	fatty acid desaturase 3	FADS3	AC004770	0.7254	Below
29	38604_at	neuropeptide Y	NPY	AI198311	0.7212	Below
30	36773_f_at	major histocompatibility complex class II DQ beta 1	HLA-DQB1	M81141	0.7197	Below
31	32562_at	endoglin Osler-Rendu-Weber syndrome 1	ENG	X72012	0.7180	Below
32	36502_at	PFTAIRE protein kinase 1	PFTK1	AB020641	0.7179	Below
33	37180_at	phospholipase C gamma 2 phosphatidylinositol-specific	PLCG2	X14034	0.7114	Below
34	38893_at	neutrophil cytosolic factor 4 40kD	NCF4	AL008637	0.7100	Below
35	387_at	cyclin-dependent kinase 9 CDC2-related kinase	CDK9	X80230	0.7024	Below
36	32035_at	Human MHC class II HLA-DRw53-associated glycoprotein beta- chain mRNA complete cds	�	M16942	0.6992	Below
37	41153_f_at	Homo sapiens alphaE-catenin (CTNNA1) gene	CTNNA1	AF102803	0.6976	Below
38	40780_at	C-terminal binding protein 2	CTBP2	AF016507	0.6976	Below
39	40775_at	integral membrane protein 2A	ITM2A	AL021786	0.6952	Above
40	39402_at	interleukin 1 beta	IL1B	M15330	0.6945	Below
41	38522_s_at	CD22 antigen	CD22	X52785	0.6945	Below
42	41166_at	immunoglobulin heavy constant mu	IGHM	X58529	0.6941	Below
43	36937_s_at	PDZ and LIM domain 1 elfin	PDLIM1	U90878	0.6937	Below
44	38833_at	Human mRNA for SB classII histocompatibility antigen alpha-chain	�	X00457	0.6925	Below
45	2047_s_at	junction plakoglobin	JUP	M23410	0.6920	Below
46	36277_at	Human membran protein (CD3-epsilon) gene, exon 9.	CD3E	M23323	0.6899	Above
47	40688_at	linker for activation of T cells	LAT	AJ223280	0.6898	Above
48	39389_at	CD9 antigen p24	CD9	M38690	0.6879	Below
49	33162_at	insulin receptor	INSR	X02160	0.6879	Below
50	31891_at	chitinase 3-like 2	CHI3L2	U58515	0.6872	Above

*TEL-AML1*
1	37780_at	piccolo presynaptic cytomatrix protein	PCLO	AB011131	0.7121	Above
2	38203_at	potassium intermediate/small conductance calcium-activated channel subfamily N member 1	KCNN1	U69883	0.7086	Above
3	36524_at	Rho guanine nucleotide exchange factor GEF 4	ARHGEF4	AB029035	0.6782	Above
4	38578_at	tumor necrosis factor receptor superfamily member 7	TNFRSF7	M63928	0.6718	Above
5	32730_at	Homo sapiens mRNA for KIAA1750 protein partial cds	�	AL080059	0.6616	Above
6	34194_at	Homo sapiens cDNA FLJ21697 fis clone COL09740	�	AL049313	0.6518	Above
7	40272_at	collapsin response mediator protein 1	CRMP1	D78012	0.6160	Above
8	41819_at	FYN-binding protein FYB-120/130	FYB	U93049	0.6058	Above
9	1488_at	protein tyrosine phosphatase receptor type K	PTPRK	L77886	0.6056	Above
10	35665_at	phosphoinositide-3-kinase class 3	PIK3C3	Z46973	0.6022	Above
11	35614_at	transcription factor-like 5 basic helix-loop-helix	TCFL5	AB012124	0.5983	Above
12	36008_at	protein tyrosine phosphatase type IVA member 3	PTP4A3	AF041434	0.5976	Above
13	35362_at	myosin X	MYO10	AB018342	0.5964	Above
14	37908_at	guanine nucleotide binding protein 11	GNG11	U31384	0.5888	Above
15	39329_at	actinin alpha 1	ACTN1	X15804	0.5840	Below
16	1936_s_at	proto-oncogene c-myc, alt. transcript 3, ORF 114	�	HG3523-HT4899	0.5761	Below
17	33690_at	Homo sapiens mRNA cDNA DKFZp434A202	DKFZp434A202	AL080190	0.5725	Above
18	39389_at	CD9 antigen p24	CD9	M38690	0.5684	Below
19	37343_at	inositol 1 4 5-triphosphate receptor type 3	ITPR3	U01062	0.5642	Above
20	1299_at	telomeric repeat binding factor 2	TERF2	X93512	0.5585	Above
21	38652_at	hypothetical protein FLJ20154	FLJ20154	AF070644	0.5563	Above
22	38763_at	(clone D21-1) L-iditol-2 dehydrogenase gene	�	L29254	0.5535	Below
23	37724_at	v-myc avian myelocytomatosis viral oncogene homolog	MYC	V00568	0.5506	Below
24	36937_s_at	PDZ and LIM domain 1 elfin	PDLIM1	U90878	0.5506	Below
25	1325_at	MAD mothers against decapentaplegic Drosophila homolog 1	MADH1	U59423	0.5482	Above
26	41549_s_at	adaptor-related protein complex 1 sigma 2 subunit	AP1S2	AF091077	0.5474	Below
27	39827_at	hypothetical protein	FLJ20500	AA522530	0.5471	Below
28	32724_at	phytanoyl-CoA hydroxylase Refsum disease	PHYH	AF023462	0.5459	Above
29	31786_at	Sam68-like phosphotyrosine protein T-STAR	T-STAR	AF051321	0.5403	Above
30	38570_at	major histocompatibility complex class II DO beta	HLA-DOB	X03066	0.5384	Above
31	39330_s_at	actinin alpha 1	ACTN1	M95178	0.5375	Below
32	36493_at	lymphocyte-specific protein 1	LSP1	M33552	0.5356	Below
33	574_s_at	caspase 1 apoptosis-related cysteine protease interleukin 1 beta convertase	CASP1	M87507	0.5336	Below
34	32224_at	KIAA0769 gene product	KIAA0769	AB018312	0.5326	Above
35	1077_at	recombination activating gene 1	RAG1	M29474	0.5302	Above
36	37280_at	MAD mothers against decapentaplegic Drosophila homolog 1	MADH1	U59912	0.5283	Above
37	41200_at	CD36 antigen collagen type I receptor thrombospondin receptor like 1	CD36L1	Z22555	0.5261	Above
38	36009_at	hypothetical protein	CL683	AF091092	0.5259	Below
39	36933_at	N-myc downstream regulated	NDRG1	D87953	0.5254	Below
40	1126_s_at	Human cell surface glycoprotein CD44 (CD44) gene, 3' end of long tailed isoform.	CD44	L05424	0.5232	Below
41	39824_at	ESTs	�	AI391564	0.5231	Above
42	38078_at	filamin B beta actin-binding protein-278	FLNB	AF042166	0.5208	Below
43	38127_at	syndecan 1	SDC1	Z48199	0.5199	Above
44	32941_at	interferon consensus sequence binding protein 1	ICSBP1	M91196	0.5195	Below
45	37276_at	IQ motif containing GTPase activating protein 2	IQGAP2	U51903	0.5191	Below
46	34768_at	DKFZP564E1962 protein	DKFZP564E1962	AL080080	0.5184	Below
47	39781_at	insulin-like growth factor-binding protein 4	IGFBP4	U20982	0.5173	Below
48	37918_at	integrin beta 2 antigen CD18 p95 lymphocyte function-associated antigen 1 macrophage antigen 1 mac-1 beta subunit	ITGB2	M15395	0.5162	Below
49	41490_at	phosphoribosyl pyrophosphate synthetase 2	PRPS2	Y00971	0.5155	Below
50	41814_at	fucosidase alpha-L- 1 tissue	FUCA1	M29877	0.5101	Above

Illustrated below are the results of a two-dimensional hierarchical clustering algorithm of the 327 diagnostic ALL cases using the top 50 probe sets for each of the 7 groups chosen by the Wilkins' metric. As some genes are chosen for more than one group, there are 304 unique probe sets represented in Figure 17.

Figure 17. Heirarchical cluster of 327 Diagnostic ALL samples using genes selected by Wilkins' metric

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